Lizarraga D, Lozano C, Briedé J J, van Delft J H, Touriño S, Centelles J J, Torres J L, Cascante M
Biochemistry and Molecular Biology Department, Biology Faculty, University of Barcelona, Spain.
FEBS J. 2007 Sep;274(18):4802-11. doi: 10.1111/j.1742-4658.2007.06010.x.
Grape (Vitis vinifera) and pine (Pinus pinaster) bark extracts are widely used as nutritional supplements. Procyanidin-rich fractions from grape and pine bark extract showing different mean degrees of polymerization, percentage of galloylation (percentage of gallate esters) and reactive oxygen species-scavenging capacity were tested on HT29 human colon cancer cells. We observed that the most efficient fractions in inhibiting cell proliferation, arresting the cell cycle in G(2) phase and inducing apoptosis were the grape fractions with the highest percentage of galloylation and mean degree of polymerization. Additionally, the antiproliferative effects of grape fractions were consistent with their oxygen radical-scavenging capacity and their ability to trigger DNA condensation-fragmentation.
葡萄（欧亚葡萄）和松树（海岸松）树皮提取物被广泛用作营养补充剂。对来自葡萄和松树皮提取物的富含原花青素的馏分进行了测试，这些馏分显示出不同的平均聚合度、没食子酰化百分比（没食子酸酯的百分比）和活性氧清除能力，测试对象为HT29人结肠癌细胞。我们观察到，在抑制细胞增殖、使细胞周期停滞在G(2)期以及诱导细胞凋亡方面最有效的馏分是没食子酰化百分比和平均聚合度最高的葡萄馏分。此外，葡萄馏分的抗增殖作用与其氧自由基清除能力以及引发DNA凝聚-断裂的能力一致。
