Hattori Koji, Uematsu Kota, Tanikake Yohei, Habata Takashi, Tanaka Yasuhito, Yajima Hiroshi, Takakura Yoshinori
Department of DAIWA HOUSE Indoor Environmental Medicine, Nara Medical University, Kashihara, Nara, Japan.
Arthritis Res Ther. 2007;9(5):R88. doi: 10.1186/ar2287.
We investigated the use of a commercial spectrocolorimeter and the application of two color models (L* a* b* colorimetric system and spectral reflectance distribution) to describe and quantify cartilage plugs in a rabbit model of osteochondral autografting. Osteochondral plugs were removed and then replaced in their original positions in Japanese white rabbits. The rabbits were sacrificed at 4 or 12 weeks after the operation and cartilage samples were assessed using a spectrocolorimeter. The samples were retrospectively divided into two groups on the basis of the histological findings (group H: hyaline cartilage, successful; group F: fibrous tissue or fibrocartilage, failure) and investigated for possible significant differences in the spectrocolorimetric analyses between the two groups. Moreover, the relationships between the spectrocolorimetric indices and the Mankin histological score were examined. In the L* a* b* colorimetric system, the L* values were significantly lower in group H than in group F (P = 0.02), whereas the a* values were significantly higher in group H than in group F (P = 0.006). Regarding the spectral reflectance distribution, the spectral reflectance percentage 470 (SRP470) values, as a coincidence index for the spectral reflectance distribution (400 to 470 nm in wavelength) of the cartilage plugs with respect to intact cartilage, were 99.8 +/- 6.7% in group H and 119.8 +/- 10.6% in group F, and the difference between these values was significant (P = 0.005). Furthermore, the a* values were significantly correlated with the histological score (P = 0.004, r = -0.76). The SRP470 values were also significantly correlated with the histological score (P = 0.01, r = 0.67). Our findings demonstrate the ability of spectrocolorimetric measurements to predict the histological findings of cartilage plugs after autologous osteochondral grafting. In particular, the a* values and SRP470 values can be used to judge the surface condition of an osteochondral plug on the basis of objective data. Therefore, spectrocolorimetry may contribute to orthopedics, rheumatology and related research in arthritis, and arthroscopic use of this method may potentially be preferable for in vivo assessment.
我们研究了使用商用分光色度计以及两种颜色模型（Lab比色系统和光谱反射率分布）来描述和量化兔自体骨软骨移植模型中的软骨栓。取出骨软骨栓，然后将其放回日本白兔的原位置。术后4周或12周处死兔子，使用分光色度计评估软骨样本。根据组织学结果将样本回顾性地分为两组（H组：透明软骨，成功；F组：纤维组织或纤维软骨，失败），并研究两组之间分光色度分析可能存在的显著差异。此外，还检查了分光色度指标与Mankin组织学评分之间的关系。在Lab比色系统中，H组的L值显著低于F组（P = 0.02），而H组的a值显著高于F组（P = 0.006）。关于光谱反射率分布，作为软骨栓相对于完整软骨的光谱反射率分布（波长400至470 nm）的符合指数，光谱反射率百分比470（SRP470）值在H组为99.8±6.7%，在F组为119.8±10.6%，这些值之间的差异具有显著性（P = 0.005）。此外，a值与组织学评分显著相关（P = 0.004，r = -0.76）。SRP470值也与组织学评分显著相关（P = 0.01，r = 0.67）。我们的研究结果表明，分光色度测量能够预测自体骨软骨移植后软骨栓的组织学结果。特别是，a值和SRP470值可用于基于客观数据判断骨软骨栓的表面状况。因此，分光色度法可能有助于骨科、风湿病学以及关节炎相关研究，并且该方法的关节镜应用可能更适合体内评估。
