Azarang Atosa, Mahmoodi Majid, Rajabalian Said, Shekari Majid Asadi, Nosratabadi Jafar, Rezaei Nima
Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Eur Cytokine Netw. 2007 Dec;18(4):210-4. doi: 10.1684/ecn.2007.0107. Epub 2007 Nov 12.
There are a few studies with conflicting results on the effects of opioids on the functioning of immune system. This study was performed to investigate the in vitro production of interferon-gamma and interleukin-10 after antigenic stimulation of cells using whole blood from opioid addicts. Blood samples were taken from 20 chronically opioid-addicted persons, who voluntarily enrolled for detoxification (10 opium and 10 heroin addicts). Blood samples were also taken from 10 healthy individuals with no history of drug abuse as the control. Cell culture was performed in a whole blood culture assay. Diluted blood samples were stimulated with phytohemagglutinin or with lipopolysaccharide and the supernatants were collected to measure cytokine production. The results demonstrated a significant decrease in interferon-gamma production and an increase in interleukin-10 secretion in heroin addicts, relative to the control group (35.9+/-26.3 versus 110.2+/-60.3 pg/mL, p<0.01 and 71.8+/-28.4 versus 17.1+/-13.5 pg/mL, p<0.01, respectively), however the changes in these values in opium addicts were not significant compared to healthy individuals. The results could suggest that opioid addiction leads to a shift in the Th1/Th2 cytokine balance of peripheral CD4+ cells towards the Th2 response, and opioid addicts demonstrate reduced mitogenic responsiveness of lymphocytes relative to healthy individuals.
关于阿片类药物对免疫系统功能的影响，有一些研究结果相互矛盾。本研究旨在利用阿片类药物成瘾者的全血，调查抗原刺激细胞后干扰素-γ和白细胞介素-10的体外产生情况。从20名自愿报名戒毒的慢性阿片类药物成瘾者（10名鸦片成瘾者和10名海洛因成瘾者）采集血样。还从10名无药物滥用史的健康个体采集血样作为对照。采用全血培养试验进行细胞培养。用植物血凝素或脂多糖刺激稀释后的血样，收集上清液以测量细胞因子的产生。结果显示，与对照组相比，海洛因成瘾者的干扰素-γ产生显著减少，白细胞介素-10分泌增加（分别为35.9±26.3对110.2±60.3 pg/mL，p<0.01；71.8±28.4对17.1±13.5 pg/mL，p<0.01），然而，与健康个体相比，鸦片成瘾者这些值的变化不显著。结果可能表明，阿片类药物成瘾导致外周CD4+细胞的Th1/Th2细胞因子平衡向Th2反应转变，并且与健康个体相比，阿片类药物成瘾者淋巴细胞的促有丝分裂反应性降低。
