Puente Pilar, Anadón Ana M, Rodero Marta, Romarís Fernanda, Ubeira Florencio M, Cuéllar Carmen
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Exp Parasitol. 2008 Feb;118(2):271-4. doi: 10.1016/j.exppara.2007.07.002. Epub 2007 Aug 3.
Anisakiosis is a nematodosis with high prevalence in Spain. In this work we (a) investigated whether a recently introduced ELISA of Anisakis simplex-specific IgE in serum suffers from cross-reactivity with other common allergens; (b) used this assay to obtain an estimate of the prevalence of A. simplex-specific IgE in the population of Madrid; and (c) related positivity to fish consumption habits. No evidence of cross-reactivity between the ELISA and other allergens was found. The prevalence of positivity was 12.4% (11.7% among healthy subjects and 16% among patients with non-digestive non-allergic pathologies). All interviewed subjects reported consumption of uncooked fish (known to be the most likely source of infection); in addition, positivity was more prevalent among subjects who habitually consumed fresh and possibly undercooked fish than among those who generally consumed frozen fish or boiled or baked fish. These results are discussed in relation to the much lower prevalence observed in Galicia (N.W. Spain).
异尖线虫病是一种在西班牙高发的线虫病。在本研究中，我们：(a) 调查了最近引入的血清中简单异尖线虫特异性IgE酶联免疫吸附测定（ELISA）是否与其他常见过敏原存在交叉反应；(b) 使用该检测方法估计马德里人群中简单异尖线虫特异性IgE的患病率；以及 (c) 将阳性结果与鱼类食用习惯相关联。未发现ELISA与其他过敏原之间存在交叉反应的证据。阳性率为12.4%（健康受试者中为11.7%，非消化性非过敏性疾病患者中为16%）。所有接受访谈的受试者均报告食用过生鱼（已知这是最可能的感染源）；此外，习惯性食用新鲜且可能未煮熟鱼类的受试者中的阳性率高于一般食用冷冻鱼或煮鱼或烤鱼的受试者。结合在加利西亚（西班牙西北部）观察到的低得多的患病率对这些结果进行了讨论。
