Altay Bariş, Turna Burak, Oktem Gülperi, Aktuğ Hüseyin, Semerci Bülent, Bilir Ayhan
Department of Urology, Ege University Medical Faculty, Izmir, Turkey.
Fertil Steril. 2008 Jul;90(1):141-7. doi: 10.1016/j.fertnstert.2007.05.065. Epub 2007 Sep 6.
To describe the effects of epididymal and vasal ligation, in an experimental rat model, by using connexin 43 and occludin immunohistochemistry as well as transmission electron microscopy.
Comparative and controlled experimental research study.
University animal research and histology laboratories in Turkey.
ANIMAL(S): Wistar male rats in experimental and control groups.
INTERVENTION(S): The control group underwent sham operation (n = 7). The first experimental group (n = 7) underwent unilateral epididymal ligation, whereas the second experimental group (n = 7) underwent unilateral vasal ligation to induce experimental epididymal and vasal obstruction models, respectively. All animals were then killed at 90 days.
MAIN OUTCOME MEASURE(S): Immunohistochemical expression of connexin 43 and occludin for testicular tissues was determined after epididymal and vasal obstruction models. Ultrastructural morphological changes were examined by electron microscopy.
RESULT(S): Results of the semiquantitative analysis revealed that expressions of both occludin and connexin 43 in the rat testis were decreased in the experimental groups compared with in the sham-operated group. However, changes after vasal ligation were more prominent. Ultrastructural examination confirmed decreased intercellular communication as well as increased cellular degeneration among the ipsilateral and contralateral testicular tissues.
CONCLUSION(S): Immunohistochemical expression of occludin and connexin 43 were decreased in the testis after vasal and epididymal ligation when compared with the sham-operated group. Ultrastructural changes indicating cell degeneration were more prominent after vasal ligation.
通过使用连接蛋白43和闭合蛋白免疫组织化学以及透射电子显微镜，描述在实验性大鼠模型中附睾和输精管结扎的影响。
比较性和对照性实验研究。
土耳其的大学动物研究和组织学实验室。
实验组和对照组的Wistar雄性大鼠。
对照组接受假手术（n = 7）。第一个实验组（n = 7）进行单侧附睾结扎，而第二个实验组（n = 7）进行单侧输精管结扎，分别诱导实验性附睾和输精管梗阻模型。然后在90天时处死所有动物。
在建立附睾和输精管梗阻模型后，测定睾丸组织中连接蛋白43和闭合蛋白的免疫组织化学表达。通过电子显微镜检查超微结构形态变化。
半定量分析结果显示，与假手术组相比，实验组大鼠睾丸中闭合蛋白和连接蛋白43的表达均降低。然而，输精管结扎后的变化更为明显。超微结构检查证实同侧和对侧睾丸组织之间的细胞间通讯减少以及细胞变性增加。
与假手术组相比，输精管和附睾结扎后睾丸中闭合蛋白和连接蛋白43的免疫组织化学表达降低。输精管结扎后表明细胞变性的超微结构变化更为明显。
