Department of Histology and Embryology, School of Medicine, Marmara University, Istanbul, Turkey.
Int J Exp Pathol. 2012 Dec;93(6):438-49. doi: 10.1111/j.1365-2613.2012.00844.x.
In utero irradiation (IR) and postnatal hyperthermia (HT) exposure cause infertility by decreasing spermatogenic colony growth and the number of sperm in rats. Four groups were used: (i) Control group, (ii) HT group (rats exposed to hyperthermia on the 10th postnatal day), (iii) IR group (rats exposed to IR on the 17th gestational day) and (iv) IR + HT group. Three and six months after the procedures testes were examined by light and electron microscopy. Some degenerated tubules in the HT group, many vacuoles in spermatogenic cells and degenerated tight junctions in the IR group, atrophic tubules and severe degeneration of tight junctions in the IR + HT group were observed. ZO-1 and occludin immunoreactivity were decreased and disorganized in the HT and IR groups and absent in the IR + HT group. The increase in the number of apoptotic cells was accompanied by a time-dependent decrease in haploid, diploid and tetraploid cells in all groups. Degenerative findings were severe after 6 months in all groups. The double-hit model may represent a Sertoli cell only model of infertility due to a decrease in spermatogenic cell and alterated blood-testis barrier proteins in rat.
宫内照射（IR）和产后高热（HT）暴露可通过减少生精细胞集落生长和精子数量导致大鼠不育。使用了四组：（i）对照组，（ii）HT 组（第 10 天产后暴露于高热），（iii）IR 组（第 17 天妊娠时暴露于 IR）和（iv）IR+HT 组。程序后 3 个月和 6 个月，通过光镜和电子显微镜检查睾丸。HT 组观察到一些退化的小管，IR 组生精细胞中有许多空泡和退化的紧密连接，IR+HT 组观察到萎缩的小管和紧密连接的严重退化。ZO-1 和闭合蛋白免疫反应性在 HT 和 IR 组中减少且紊乱，在 IR+HT 组中缺失。凋亡细胞数量的增加伴随着所有组中单倍体、二倍体和四倍体细胞的时间依赖性减少。所有组在 6 个月后退行性发现更为严重。双打击模型可能代表由于生精细胞减少和大鼠血睾屏障蛋白改变导致的 Sertoli 细胞不育的唯一模型。
