Sahin Zeliha, Celik-Ozenci Ciler, Akkoyunlu Gokhan, Korgun Emin Turkay, Acar Nuray, Erdogru Tibet, Demir Ramazan, Ustunel Ismail
Department of Histology and Embryology, Akdeniz University, Faculty of Medicine, Antalya, Turkey.
Fertil Steril. 2006 Apr;85 Suppl 1:1265-75. doi: 10.1016/j.fertnstert.2005.10.025.
To describe the effect of varicocele, in an experimental rat model, on the levels of IL-1alpha and IL-1beta proteins in testis tissue.
Comparative and controlled study.
Experimental research.
ANIMAL(S): Wistar male rats in experimental and control groups.
INTERVENTION(S): The control group underwent sham operation (n = 6). Experimental groups underwent partial ligation of the renal vein to induce experimental varicocele and were then killed at 9 (n = 6), 11 (n = 6), and 13 (n = 6) weeks after induction of varicocele.
MAIN OUTCOME MEASURE(S): Histologic evaluation of the varicocele model was determined by periodic acid-Schiff staining of paraffin-embeded testicular tissues. Levels of cytokines were assessed by immunohistochemistry and Western blot analysis.
RESULT(S): Varicocele caused testicular damage, especially in 11- and 13-week-old varicocele groups. In sham-operated rats, Golgi complexes of round spermatids expressed especially the alpha form of IL-1. By the progression of varicocele, the IL-1alpha expression increased temporally in Sertoli cells, spermatogonia, primary spermatocytes, spermatids, and Leydig cells. The expression of IL-1beta was seen in Leydig cells in sham-operated rats. The IL-1beta expression was also increased upon progression of varicocele in Leydig cells, Sertoli cells, and spermatogonia.
CONCLUSION(S): We suggest that IL-1alpha and IL-1beta are the regulators of testicular function. Certain pathologic conditions, e.g., varicocele, cause an increase in the expressions of such proinflammatory cytokines. The increased expression of IL-1alpha and IL-1beta in varicocele shifts the balance in favor of inflammatory and immune responses and causes detrimental effects in testis tissue, which may cause male infertility.
在实验大鼠模型中描述精索静脉曲张对睾丸组织中白细胞介素-1α（IL-1α）和白细胞介素-1β（IL-1β）蛋白水平的影响。
比较性对照研究。
实验研究。
实验组和对照组的雄性Wistar大鼠。
对照组接受假手术（n = 6）。实验组进行肾静脉部分结扎以诱导实验性精索静脉曲张，然后在精索静脉曲张诱导后9周（n = 6）、11周（n = 6）和13周（n = 6）处死。
通过对石蜡包埋的睾丸组织进行高碘酸-希夫染色来确定精索静脉曲张模型的组织学评估。通过免疫组织化学和蛋白质印迹分析评估细胞因子水平。
精索静脉曲张导致睾丸损伤，尤其是在11周和13周龄的精索静脉曲张组。在假手术大鼠中，圆形精子细胞的高尔基体特别表达IL-1的α形式。随着精索静脉曲张的进展，IL-1α在支持细胞、精原细胞、初级精母细胞、精子细胞和间质细胞中的表达随时间增加。在假手术大鼠的间质细胞中可见IL-1β的表达。随着精索静脉曲张的进展，IL-1β在间质细胞、支持细胞和精原细胞中的表达也增加。
我们认为IL-1α和IL-1β是睾丸功能的调节因子。某些病理状况，如精索静脉曲张，会导致此类促炎细胞因子的表达增加。精索静脉曲张中IL-1α和IL-1β表达的增加使平衡向有利于炎症和免疫反应的方向转变，并对睾丸组织产生有害影响，这可能导致男性不育。
