Kajiume Teruyuki, Yuge Louis, Kawahara Yumi, Yoshimoto Reiko, Sasaki Akira, Ide Toshinori, Asashima Makoto, Kataoka Katsuko, Kobayashi Masao
Department of Pediatrics, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
FEBS Lett. 2007 Oct 2;581(24):4645-50. doi: 10.1016/j.febslet.2007.08.057. Epub 2007 Sep 7.
In this study we examined the effect of the specific gravity of culture medium on the frequency of hematopoietic stem cell (HSC) maintenance. We used a newly developed high-specific-gravity media. Bone marrow cells were isolated and cultured, and HSC activity was evaluated. The number of hematopoietic progenitor/stem cells was markedly higher in the medium with high specific gravity. In high-specific-gravity media, cells did not precipitate, maintenance of HSCs was increased, and there was a concomitant accumulation of beta-catenin. This novel technique for maintaining HSC populations provides an important new tool for studies in regenerative medicine.
