Chu Chun-Yen, Wang Shiang-Yiu, Chen Zeng-Weng, Chien Maw-Sheng, Huang Ji-Ping, Chen Jen-Jin, Hong Li-Shian, Shiau Ai-Li, Tsai Jeng-Liang, Wu Chao-Liang
Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, 1 Shuehfu Road, Neipu, Pingtung 91201, Taiwan.
Vaccine. 2007 Oct 10;25(41):7031-40. doi: 10.1016/j.vaccine.2007.07.063. Epub 2007 Aug 21.
In this study, we exploited a crp (cAMP receptor protein) gene-deleted, virulence plasmid-cured Salmonella choleraesuis mutant with decreased carbon source utilization, designated S.C.-Deltacrp/vpl(-), as a live vaccine strain. Normal weight gain with no clinical signs was observed in pigs immunized with high doses of S.C.-Deltacrp/vpl(-) live vaccine. Vaccination in pregnant sows induced high maternal antibodies, which could prevent piglets from Salmonella infection. Moreover, serial transmission of the vaccine strain in piglets produced no evidence of reversion to virulence. Furthermore, the peripheral blood mononuclear cells from immunized piglets also developed Salmonella specific T-cell proliferative response in vitro. Our results indicate that immunogenic antigens in S.C.-Deltacrp/vpl(-) can induce adequate immunity to protect pigs against challenge with a heterologous virulent strain. Thus, this mutant holds promise for the development of a new live S. choleraesuis vaccine.
在本研究中，我们利用了一株碳源利用能力降低的环磷酸腺苷受体蛋白（crp）基因缺失、毒力质粒清除的猪霍乱沙门氏菌突变株，命名为S.C.-Deltacrp/vpl(-)，作为一种活疫苗株。用高剂量S.C.-Deltacrp/vpl(-)活疫苗免疫的猪体重正常增加，无临床症状。给怀孕母猪接种疫苗可诱导产生高水平的母源抗体，从而预防仔猪感染沙门氏菌。此外，疫苗株在仔猪中的连续传代未出现毒力回复的迹象。此外，免疫仔猪的外周血单核细胞在体外也产生了沙门氏菌特异性T细胞增殖反应。我们的结果表明，S.C.-Deltacrp/vpl(-)中的免疫原性抗原可诱导足够的免疫力，以保护猪免受异源强毒株的攻击。因此，该突变株有望用于开发新型猪霍乱沙门氏菌活疫苗。
