• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

携带有猪霍乱沙门氏菌 O 多糖异源表达的重组减毒鼠伤寒沙门氏菌：高免疫原性和保护作用。

Recombinant attenuated Salmonella Typhimurium with heterologous expression of the Salmonella Choleraesuis O-polysaccharide: high immunogenicity and protection.

机构信息

Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.

Key Laboratory of Animal Disease and Human Health of Sichuan Province, Wenjiang, Chengdu, Sichuan, 611130, P.R. China.

出版信息

Sci Rep. 2017 Jul 28;7(1):7127. doi: 10.1038/s41598-017-07689-5.

DOI:10.1038/s41598-017-07689-5
PMID:28754982
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5533773/
Abstract

Non-typhoidal Salmonella are associated with gastrointestinal disease worldwide and invasive disease in Africa. We constructed novel bivalent vaccines through the recombinant expression of heterologous O-antigens from Salmonella Choleraesuis in Salmonella Typhimurium. A recombinant Asd plasmid pCZ1 with the cloned Salmonella Choleraesuis O-antigen gene cluster was introduced into three constructed Salmonella Typhimurium Δasd mutants: SLT11 (ΔrfbP), SLT12 (ΔrmlB-rfbP) and SLT16 (ΔrfbP ∆pagL::TT araCP rfbP). Immunoblotting demonstrated that SLT11 (pCZ1) and SLT12 (pCZ1) efficiently expressed the heterologous O-antigen. In the presence of arabinose, SLT16 (pCZ1) expressed both the homologous and heterologous O-antigens, whereas in the absence of arabinose, SLT16 (pCZ1) mainly expressed the heterologous O-antigen. We deleted the crp/cya genes in SLT12 (pCZ1) and SLT16 (pCZ1) for attenuation purposes, generating the recombinant vaccine strains SLT17 (pCZ1) and SLT18 (pCZ1). Immunization with either SLT17 (pCZ1) or SLT18 (pCZ1) induced specific IgG against the heterologous O-antigen, which mediated significant killing of Salmonella Choleraesuis and provided full protection against a lethal homologous challenge in mice. Furthermore, SLT17 (pCZ1) or SLT18 (pCZ1) immunization resulted in 83% or 50% heterologous protection against Salmonella Choleraesuis challenge, respectively. Our study demonstrates that heterologous O-antigen expression is a promising strategy for the development of multivalent Salmonella vaccines.

摘要

非伤寒沙门氏菌与全世界的胃肠道疾病和非洲的侵袭性疾病有关。我们通过在鼠伤寒沙门氏菌中重组表达猪霍乱沙门氏菌的异源 O 抗原，构建了新型双价疫苗。带有克隆的猪霍乱沙门氏菌 O 抗原基因簇的重组 Asd 质粒 pCZ1 被引入到三个构建的鼠伤寒沙门氏菌Δasd 突变株中：SLT11（ΔrfbP）、SLT12（ΔrmlB-rfbP）和 SLT16（ΔrfbPΔpagL::TT araCP rfbP）。免疫印迹表明，SLT11（pCZ1）和 SLT12（pCZ1）有效地表达了异源 O 抗原。在阿拉伯糖存在的情况下，SLT16（pCZ1）表达同源和异源 O 抗原，而在没有阿拉伯糖的情况下，SLT16（pCZ1）主要表达异源 O 抗原。我们为了减毒的目的在 SLT12（pCZ1）和 SLT16（pCZ1）中删除了 crp/cya 基因，产生了重组疫苗株 SLT17（pCZ1）和 SLT18（pCZ1）。用 SLT17（pCZ1）或 SLT18（pCZ1）免疫诱导了针对异源 O 抗原的特异性 IgG，该抗体介导了对猪霍乱沙门氏菌的显著杀伤，并在小鼠中提供了针对同源致命挑战的完全保护。此外，SLT17（pCZ1）或 SLT18（pCZ1）免疫分别导致对猪霍乱沙门氏菌攻击的 83%或 50%异源保护。我们的研究表明，异源 O 抗原表达是开发多价沙门氏菌疫苗的有前途的策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/46648bc9b114/41598_2017_7689_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/12c194b4624a/41598_2017_7689_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/32722922a064/41598_2017_7689_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/0c86c49e33e8/41598_2017_7689_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/a8dfcbf2389a/41598_2017_7689_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/fb2fad324ed7/41598_2017_7689_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/46648bc9b114/41598_2017_7689_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/12c194b4624a/41598_2017_7689_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/32722922a064/41598_2017_7689_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/0c86c49e33e8/41598_2017_7689_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/a8dfcbf2389a/41598_2017_7689_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/fb2fad324ed7/41598_2017_7689_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f45a/5533773/46648bc9b114/41598_2017_7689_Fig6_HTML.jpg

相似文献

1
Recombinant attenuated Salmonella Typhimurium with heterologous expression of the Salmonella Choleraesuis O-polysaccharide: high immunogenicity and protection.携带有猪霍乱沙门氏菌 O 多糖异源表达的重组减毒鼠伤寒沙门氏菌：高免疫原性和保护作用。
Sci Rep. 2017 Jul 28;7(1):7127. doi: 10.1038/s41598-017-07689-5.
2
Regulated delayed attenuation enhances the immunogenicity and protection provided by recombinant Salmonellaenterica serovar Typhimurium vaccines expressing serovar Choleraesuis O-polysaccharides.调控延迟衰减增强了表达血清型霍乱弧菌 O 多糖的重组鼠伤寒沙门氏菌疫苗的免疫原性和保护作用。
Vaccine. 2018 Aug 9;36(33):5010-5019. doi: 10.1016/j.vaccine.2018.07.009. Epub 2018 Jul 13.
3
Two Novel Bivalent Vaccines Confer Dual Protection against Two Serovars in Mice.两种新型二价疫苗在小鼠中提供针对两种血清型的双重保护。
Front Cell Infect Microbiol. 2017 Sep 4;7:391. doi: 10.3389/fcimb.2017.00391. eCollection 2017.
4
Regulated delayed synthesis of lipopolysaccharide and enterobacterial common antigen of Salmonella Typhimurium enhances immunogenicity and cross-protective efficacy against heterologous Salmonella challenge.鼠伤寒沙门氏菌脂多糖和肠杆菌共同抗原的调控延迟合成增强了对异源沙门氏菌攻击的免疫原性和交叉保护效力。
Vaccine. 2016 Aug 5;34(36):4285-92. doi: 10.1016/j.vaccine.2016.07.010.
5
Regulated delayed attenuation improves vaccine efficacy in preventing infection from avian pathogenic Escherichia coli O and Salmonella typhimurium.调控性延迟衰减可提高疫苗预防禽致病性大肠杆菌 O 和鼠伤寒沙门氏菌感染的效力。
Vet Microbiol. 2021 Mar;254:109012. doi: 10.1016/j.vetmic.2021.109012. Epub 2021 Feb 6.
6
A bivalent vaccine derived from attenuated Salmonella expressing O-antigen polysaccharide provides protection against avian pathogenic Escherichia coli O1 and O2 infection.一种减毒沙门氏菌表达 O 抗原多糖的二价疫苗可提供针对禽致病性大肠杆菌 O1 和 O2 感染的保护。
Vaccine. 2018 Feb 14;36(8):1038-1046. doi: 10.1016/j.vaccine.2018.01.036. Epub 2018 Jan 19.
7
Live-attenuated Salmonella enterica serotype Choleraesuis vaccine with regulated delayed fur mutation confer protection against Streptococcus suis in mice.具有调控延迟突变的减毒猪霍乱沙门氏菌疫苗对小鼠抗猪链球菌的保护作用。
BMC Vet Res. 2020 May 7;16(1):129. doi: 10.1186/s12917-020-02340-4.
8
Reversible synthesis of colanic acid and O-antigen polysaccharides in Salmonella Typhimurium enhances induction of cross-immune responses and provides protection against heterologous Salmonella challenge.鼠伤寒沙门氏菌中结肠酸和O抗原多糖的可逆合成增强了交叉免疫反应的诱导，并提供了针对异源沙门氏菌攻击的保护作用。
Vaccine. 2017 May 15;35(21):2862-2869. doi: 10.1016/j.vaccine.2017.04.002. Epub 2017 Apr 12.
9
Heterologous Pseudomonas aeruginosa O-antigen delivery using a Salmonella enterica serovar Typhimurium wecA mutant strain.利用肠炎沙门氏菌鼠伤寒血清型wecA突变株进行异源铜绿假单胞菌O抗原传递。
Int J Med Microbiol. 2016 Nov;306(7):529-540. doi: 10.1016/j.ijmm.2016.06.005. Epub 2016 Jun 28.
10
[Characterization of attenuated Salmonella C500 strain with a delta asd mutant and use as an Asd+ balanced-lethal host-vector system].[具有Δasd突变体的减毒沙门氏菌C500菌株的特性鉴定及其作为Asd +平衡致死宿主-载体系统的应用]
Sheng Wu Gong Cheng Xue Bao. 2009 Jan;25(1):29-36.

引用本文的文献

1
Roles of SPI-2 T3SS effectors in virulence of Choleraesuis and Construction of a triple-gene mutant vaccine strain.SPI-2三型分泌系统效应蛋白在猪霍乱沙门氏菌毒力中的作用及三基因缺失疫苗株的构建
Front Vet Sci. 2025 Aug 12;12:1637327. doi: 10.3389/fvets.2025.1637327. eCollection 2025.
2
A Listeria ivanovii balanced-lethal system may be a promising antigen carrier for vaccine construction.李斯特菌（Listeria ivanovii）平衡致死系统可能是疫苗构建有前途的抗原载体。
Microb Biotechnol. 2022 Nov;15(11):2831-2844. doi: 10.1111/1751-7915.14137. Epub 2022 Sep 7.
3
Immunogenicity and protection of a Pasteurella multocida strain with a truncated lipopolysaccharide outer core in ducks.

本文引用的文献

1
Mucosal IgA and IFN-γ CD8 T cell immunity are important in the efficacy of live Salmonella enteria serovar Choleraesuis vaccines.黏膜 IgA 和 IFN-γ CD8 T 细胞免疫在活伤寒沙门氏菌血清型疫苗的功效中很重要。
Sci Rep. 2017 Apr 13;7:46408. doi: 10.1038/srep46408.
2
Regulated delayed synthesis of lipopolysaccharide and enterobacterial common antigen of Salmonella Typhimurium enhances immunogenicity and cross-protective efficacy against heterologous Salmonella challenge.鼠伤寒沙门氏菌脂多糖和肠杆菌共同抗原的调控延迟合成增强了对异源沙门氏菌攻击的免疫原性和交叉保护效力。
Vaccine. 2016 Aug 5;34(36):4285-92. doi: 10.1016/j.vaccine.2016.07.010.
3
鸭源缺失脂多糖外核心的多杀性巴氏杆菌菌株的免疫原性和保护作用。
Vet Res. 2022 Mar 2;53(1):17. doi: 10.1186/s13567-022-01035-y.
4
Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickens.一株表面展示艾美耳球虫 AMA1 和 EtMIC2 蛋白的食品级重组植物乳杆菌对肉鸡的保护作用。
Microb Cell Fact. 2020 Feb 11;19(1):28. doi: 10.1186/s12934-020-1297-4.
Immunogenicity and Cross-Protective Efficacy Induced by Outer Membrane Proteins from Salmonella Typhimurium Mutants with Truncated LPS in Mice.
鼠伤寒沙门氏菌LPS截短突变体的外膜蛋白在小鼠体内诱导的免疫原性和交叉保护效力
Int J Mol Sci. 2016 Mar 22;17(3):416. doi: 10.3390/ijms17030416.
4
Identification of the Avian Pasteurella multocida phoP Gene and Evaluation of the Effects of phoP Deletion on Virulence and Immunogenicity.禽多杀性巴氏杆菌phoP基因的鉴定及phoP基因缺失对毒力和免疫原性影响的评估
Int J Mol Sci. 2015 Dec 23;17(1):12. doi: 10.3390/ijms17010012.
5
Live-Attenuated Bacterial Vectors: Tools for Vaccine and Therapeutic Agent Delivery.活减细菌性载体：疫苗和治疗剂传递工具。
Vaccines (Basel). 2015 Nov 10;3(4):940-72. doi: 10.3390/vaccines3040940.
6
Recombinant Salmonella Expressing Burkholderia mallei LPS O Antigen Provides Protection in a Murine Model of Melioidosis and Glanders.表达类鼻疽伯克霍尔德菌脂多糖O抗原的重组沙门氏菌在类鼻疽和鼻疽小鼠模型中提供保护作用。
PLoS One. 2015 Jul 6;10(7):e0132032. doi: 10.1371/journal.pone.0132032. eCollection 2015.
7
Serotypes, seasonal trends, and antibiotic resistance of non-typhoidal Salmonella from human patients in Guangdong Province, China, 2009-2012.2009 - 2012年中国广东省人类患者非伤寒沙门氏菌的血清型、季节性趋势及抗生素耐药性
BMC Infect Dis. 2015 Feb 12;15:53. doi: 10.1186/s12879-015-0784-4.
8
Design of glycoconjugate vaccines against invasive African Salmonella enterica serovar Typhimurium.针对侵袭性非洲肠炎沙门氏菌鼠伤寒血清型的糖缀合物疫苗设计。
Infect Immun. 2015 Mar;83(3):996-1007. doi: 10.1128/IAI.03079-14. Epub 2014 Dec 29.
9
Vaccines against invasive Salmonella disease: current status and future directions.抗侵袭性沙门氏菌病疫苗：现状与未来方向
Hum Vaccin Immunother. 2014;10(6):1478-93. doi: 10.4161/hv.29054. Epub 2014 May 7.
10
Out of Africa: links between invasive nontyphoidal Salmonella disease, typhoid fever, and malaria.走出非洲：侵袭性非伤寒沙门氏菌病、伤寒热和疟疾之间的联系。
Clin Infect Dis. 2014 Mar;58(5):648-50. doi: 10.1093/cid/cit803. Epub 2013 Dec 13.