酿酒酵母DNA复制突变体cdc8中的化学诱变与DNA合成

Chemical mutagenesis and DNA synthesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae.

作者信息

Baranowska H, Zuk J

机构信息

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.

出版信息

Curr Genet. 1991 Dec;20(6):471-4. doi: 10.1007/BF00334774.

DOI:10.1007/BF00334774

PMID:1782675

Abstract

Incubation of cdc8 mutants of the yeast Saccharomyces cerevisiae in YPD under permissive conditions, when DNA replication is taking place, prior to transfer to restrictive conditions, strongly stimulates induction of cdc+ colonies of ethyl methane sulphonate (EMS)- and methyl methane sulphonate (MMS)-treated yeast strains HB23 (cdc8-1/cdc8-3), HB26 (cdc8-3/cdc8-3) and HB7 (cdc8-1/cdc8-1). After diepoxybutane (DEB) treatment, both the induction of cdc+ colonies and their stimulation after incubation in YPD under permissive conditions is low. The results obtained show that stimulation of induction of cdc+ colonies under permissive conditions occurs not only after UV-treatment, but also after treatment with such mutagens as EMS and MMS.

摘要

在允许条件下（即DNA复制正在进行时），将酿酒酵母的cdc8突变体在YPD中培养，然后再转移到限制条件下，这会强烈刺激经甲磺酸乙酯（EMS）和甲磺酸甲酯（MMS）处理的酵母菌株HB23（cdc8 - 1/cdc8 - 3）、HB26（cdc8 - 3/cdc8 - 3）和HB7（cdc8 - 1/cdc8 - 1）产生cdc⁺菌落。在用1,4 - 丁二醇二缩水甘油醚（DEB）处理后，cdc⁺菌落的诱导以及在允许条件下于YPD中培养后的刺激作用都很低。所得结果表明，在允许条件下对cdc⁺菌落诱导的刺激不仅发生在紫外线处理后，也发生在用EMS和MMS等诱变剂处理后。