CDC8基因在酿酒酵母DNA单链断裂修复中的作用。
Role of the CDC8 gene in the repair of single strand breaks in DNA of the yeast Saccharomyces cerevisiae.
作者信息
Baranowska H, Zaborowska D, Jachymczyk W J, Zuk J
机构信息
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
出版信息
Curr Genet. 1990 Oct;18(3):175-9. doi: 10.1007/BF00318376.
It has been found that the repair of single strand breaks is defective in the DNA replication mutants cdc8-1 and cdc8-3 of Saccharomyces cerevisiae both in permissive (23 degrees C) and restrictive conditions (36 degrees C). In permissive conditions we observed a significant delay in single strand break repair in a diploid strain HB7 (cdc8-1/cdc8-1), as compared with the wild-type strain. Under restrictive conditions no repair was observed, but rather degradation of MMS-damaged DNA occurred. It has been also found that the repair of single strand breaks in yeast is inhibited by cycloheximide but not by hydroxyurea.
已发现，在允许条件（23摄氏度）和限制条件（36摄氏度）下，酿酒酵母的DNA复制突变体cdc8 - 1和cdc8 - 3中，单链断裂的修复存在缺陷。在允许条件下，与野生型菌株相比，我们观察到二倍体菌株HB7（cdc8 - 1/cdc8 - 1）中单链断裂修复出现显著延迟。在限制条件下，未观察到修复，而是发生了甲磺酸甲酯（MMS）损伤DNA的降解。还发现酵母中单链断裂的修复受到环己酰亚胺的抑制，但不受羟基脲的抑制。
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