DNA复制对酿酒酵母CDC8基因突变的影响。
The effect of DNA replication on mutation of the Saccharomyces cerevisiae CDC8 gene.
作者信息
Zaborowska D, Zuk J
机构信息
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
出版信息
Curr Genet. 1990 Apr;17(4):275-80. doi: 10.1007/BF00314872.
Incubation in YPD medium under permissive conditions when DNA replication is going on, strongly stimulates the induction of cdc+ colonies of UV-irradiated cells of yeast strains HB23 (cdc8-1/cdc8-3), HB26 (cdc8-3/cdc8-3) and HB7 (cdc8-1/cdc8-1). Inhibition of DNA replication by hydroxyurea, araCMP, cycloheximide or caffeine or else by incubation in phosphate buffer pH 7.0, abolishes this stimulation. Thus the replication of DNA is strongly correlated with the high induction of cdc+ colonies by UV irradiation. It is postulated that these UV-induced cdc+ colonies arise as the result infidelity in DNA replication.
在允许条件下于YPD培养基中培养，此时DNA复制正在进行，这会强烈刺激酵母菌株HB23（cdc8 - 1/cdc8 - 3）、HB26（cdc8 - 3/cdc8 - 3）和HB7（cdc8 - 1/cdc8 - 1）经紫外线照射的细胞中cdc⁺菌落的诱导。羟基脲、araCMP、环己酰亚胺或咖啡因对DNA复制的抑制，或者在pH 7.0的磷酸盐缓冲液中培养，都会消除这种刺激。因此，DNA复制与紫外线照射诱导的cdc⁺菌落的高诱导率密切相关。据推测，这些紫外线诱导的cdc⁺菌落是DNA复制中出现错误的结果。
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