Chevolleau Sylvie, Jacques Carine, Canlet Cécile, Tulliez Jacques, Debrauwer Laurent
UMR1089 Xénobiotiques, INRA-ENVT, BP 3, F-31931 Toulouse, France.
J Chromatogr A. 2007 Oct 12;1167(2):125-34. doi: 10.1016/j.chroma.2007.07.044. Epub 2007 Jul 28.
The determination of biomarkers of acrylamide exposure in humans from general French population by measurement of hemoglobin adduct levels of acrylamide (AA) and glycidamide (GA) is presented. The analytical procedure included modified Edman degradation and LC-ESI-MS/MS analysis of the final derivatives using deuterated internal standards. Method performances were evaluated in terms of linearity, precision, accuracy, and sensitivity. The method was firstly assessed on rat blood samples and then applied to the study of background adducts levels of AA and GA in 68 human hemoglobin samples, showing mean levels of 33 and 23 pmol/g globin for AA and GA adducts, respectively.
本文介绍了通过测量丙烯酰胺（AA）和环氧丙酰胺（GA）的血红蛋白加合物水平来确定法国普通人群中人类丙烯酰胺暴露生物标志物的方法。分析程序包括改良的埃德曼降解法以及使用氘代内标对最终衍生物进行液相色谱-电喷雾串联质谱（LC-ESI-MS/MS）分析。从线性、精密度、准确度和灵敏度方面对方法性能进行了评估。该方法首先在大鼠血样上进行评估，然后应用于对68份人类血红蛋白样本中AA和GA背景加合物水平的研究，结果显示AA和GA加合物的平均水平分别为33和23 pmol/g珠蛋白。
