Gordon Sean P, Heisler Marcus G, Reddy G Venugopala, Ohno Carolyn, Das Pradeep, Meyerowitz Elliot M
Division of Biology, California Institute of Technology, Pasadena, CA, USA.
Development. 2007 Oct;134(19):3539-48. doi: 10.1242/dev.010298.
Most multicellular organisms have a capacity to regenerate tissue after wounding. Few, however, have the ability to regenerate an entire new body from adult tissue. Induction of new shoot meristems from cultured root explants is a widely used, but poorly understood, process in which apical plant tissues are regenerated from adult somatic tissue through the de novo formation of shoot meristems. We characterize early patterning during de novo development of the Arabidopsis shoot meristem using fluorescent reporters of known gene and protein activities required for shoot meristem development and maintenance. We find that a small number of progenitor cells initiate development of new shoot meristems through stereotypical stages of reporter expression and activity of CUP-SHAPED COTYLEDON 2 (CUC2), WUSCHEL (WUS), PIN-FORMED 1 (PIN1), SHOOT-MERISTEMLESS (STM), FILAMENTOUS FLOWER (FIL, also known as AFO), REVOLUTA (REV), ARABIDOPSIS THALIANA MERISTEM L1 LAYER (ATML1) and CLAVATA 3 (CLV3). Furthermore, we demonstrate a functional requirement for WUS activity during de novo shoot meristem initiation. We propose that de novo shoot meristem induction is an easily accessible system for the study of patterning and self-organization in the well-studied model organism Arabidopsis.
大多数多细胞生物在受伤后都有再生组织的能力。然而，很少有生物能够从成体组织再生出全新的身体。从培养的根外植体诱导新的茎尖分生组织是一个广泛应用但了解甚少的过程，在此过程中，顶端植物组织通过茎尖分生组织的从头形成从成体体细胞组织再生而来。我们使用茎尖分生组织发育和维持所需的已知基因和蛋白质活性的荧光报告基因，对拟南芥茎尖分生组织从头发育过程中的早期模式进行了表征。我们发现，少数祖细胞通过报告基因表达的定型阶段以及杯状子叶2（CUC2）、WUSCHEL（WUS）、PIN-FORMED 1（PIN1）、无茎尖分生组织（STM）、丝状花（FIL，也称为AFO）、REVOLUTA（REV）、拟南芥分生组织L1层（ATML1）和CLAVATA 3（CLV3）的活性启动新茎尖分生组织的发育。此外，我们证明了WUS活性在从头茎尖分生组织起始过程中的功能需求。我们提出，从头茎尖分生组织诱导是一个易于研究的系统，可用于研究在深入研究的模式生物拟南芥中的模式形成和自我组织。
