在空气应激期间使用无创血压测量法测量大鼠的升压反应。

The use of non-invasive blood pressure measurements to measure pressor responses in rats during air stress.

Spanos H G, DiNicolantonio R, Morgan T O

Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.

Clin Exp Pharmacol Physiol. 1991 Nov;18(11):761-5. doi: 10.1111/j.1440-1681.1991.tb01394.x.

Abstract

A non-invasive tail cuff method was validated against direct intra-arterial blood pressure measurements (r2 greater than 0.9) and then used to measure the systolic blood pressure (SBP) responses to air-jet stimulation in mature conscious Sprague-Dawley (SD) and spontaneously hypertensive rats (SHR). The experiments with the SHR were conducted in parallel with age matched SD rats. 2. All rats were trained to remain in perspex holders and when their SBP had stabilized, a jet of air of 10 min duration was directed at the rat's nose via rubber tubing. The blood pressure was measured during the first, fifth and tenth minute. 3. The maximum SBP response normally occurred during the first min of air-jet stimulation and averaged +30 mmHg in the SHR and +21 mmHg in the SD. Although of higher magnitude in the SHR, when expressed as a percentage of the control SBP, the rise was similar for both strains, 13% and 14%, respectively. 4. The pressor response of three SD rats stimulated daily for up to 12 days did not show any evidence of habituation to the stimulus. 5. The pressor responses to air-jet stimulation were abolished in anaesthetized rats, suggesting that they are primarily higher centre responses.

摘要

一种无创尾套法经与直接动脉内血压测量验证（r2大于0.9），随后用于测量成年清醒斯普拉格-道利（SD）大鼠和自发性高血压大鼠（SHR）对喷气刺激的收缩压（SBP）反应。对SHR进行的实验与年龄匹配的SD大鼠平行进行。2. 所有大鼠均经训练以保持在有机玻璃固定器中，当其SBP稳定后，通过橡胶管将持续10分钟的气流喷向大鼠鼻部。在第1、5和10分钟测量血压。3. 最大SBP反应通常在喷气刺激的第1分钟出现，SHR平均升高30 mmHg，SD大鼠平均升高21 mmHg。尽管SHR中升高幅度更大，但以对照SBP的百分比表示时，两种品系的升高相似，分别为13%和14%。4. 对三只每天刺激长达12天的SD大鼠的升压反应未显示出对刺激有任何习惯化的迹象。5. 麻醉大鼠对喷气刺激的升压反应消失，表明它们主要是高级中枢反应。