经胃内给予表达猪细小病毒VP2蛋白的干酪乳杆菌后小鼠免疫反应的诱导
Induction of immune responses in mice after intragastric administration of Lactobacillus casei producing porcine parvovirus VP2 protein.
作者信息
Xu Yigang, Li Yijing
机构信息
Veterinary Department, Northeast Agricultural University, Harbin 150030, People's Republic of China.
出版信息
Appl Environ Microbiol. 2007 Nov;73(21):7041-7. doi: 10.1128/AEM.00436-07. Epub 2007 Sep 7.
Abstract
Lactobacillus casei ATCC 393 was selected as an antigen delivery vehicle for mucosal immunization against porcine parvovirus (PPV) infection. A 64-kDa fragment of PPV major protective antigen VP2 protein was used as the parvovirus antigen model. A recombinant Lactobacillus expressing VP2 protein was constructed with plasmid pPG611.1, where expression and localization of the VP2 protein from recombinant Lc393-rPPV-VP2 was detected via sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blotting, and immunofluorescence. Both local mucosal and systemic immune responses against PPV were induced in BALB/c mice immunized orally with the recombinant Lactobacillus expressing VP2 protein. The induced antibodies demonstrated neutralizing effects on PPV infection. These data indicated that the use of recombinant lactobacilli could be a valuable strategy for future vaccine development of PPV.
摘要
干酪乳杆菌ATCC 393被选作一种抗原递送载体，用于针对猪细小病毒（PPV）感染进行黏膜免疫。PPV主要保护性抗原VP2蛋白的一个64 kDa片段被用作细小病毒抗原模型。用质粒pPG611.1构建了表达VP2蛋白的重组乳杆菌，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、蛋白质印迹法和免疫荧光检测重组Lc393-rPPV-VP2中VP2蛋白的表达和定位。用表达VP2蛋白的重组乳杆菌经口免疫BALB/c小鼠后，诱导了针对PPV的局部黏膜和全身免疫反应。诱导产生的抗体对PPV感染具有中和作用。这些数据表明，使用重组乳杆菌可能是未来PPV疫苗开发的一种有价值的策略。
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