• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

比较经口免疫表达猪细小病毒 VP2 的干酪乳杆菌和 VP2 融合大肠杆菌不耐热肠毒素 B 亚单位蛋白诱导小鼠免疫应答的效果。

Comparison of the immune responses induced by oral immunization of mice with Lactobacillus casei-expressing porcine parvovirus VP2 and VP2 fused to Escherichia coli heat-labile enterotoxin B subunit protein.

机构信息

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, PR China.

出版信息

Comp Immunol Microbiol Infect Dis. 2011 Jan;34(1):73-81. doi: 10.1016/j.cimid.2010.02.004. Epub 2010 Mar 11.

DOI:10.1016/j.cimid.2010.02.004
PMID:20226529
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7112623/
Abstract

The major structural protein VP2 of porcine parvovirus (PPV) was used as the model parvovirus antigen, which has been expressed in Lactobacillus casei fusing with Escherichia coli heat-labile enterotoxin B subunit (LTB) as mucosal adjuvant. The VP2-LTB DNA fragment was cloned into vector pPG611 or pPG612 to generated inducible surface-displayed and secretion expression systems based on xylose promoter, designated as rLc:pPG611-VP2-LTB (recombinant L. casei) and rLc:pPG612-VP2-LTB, respectively. Expression of the fusion protein was verified by SDS-PAGE, Western blot immunofluorescence and electron microscopy. It was observed that the level of IgG or sIgA from mice orally immunized with VP2-LTB was higher than that from mice received VP2 and negative control, which demonstrated significantly statistically different. Especially, the titer of IgG or sIgA in mice immunized with rLc:pPG612-VP2-LTB is the highest in this study. In summary, LTB as mucosal adjuvant was able to effectively facilitate induction of mucosal and systemic immunity by L. casei-expressing VP2 fusion protein.

摘要

猪细小病毒（PPV）的主要结构蛋白 VP2 被用作模式细小病毒抗原，与大肠杆菌不耐热肠毒素 B 亚单位（LTB）融合在乳杆菌中表达作为黏膜佐剂。VP2-LTB DNA 片段被克隆到载体 pPG611 或 pPG612 中，基于木糖启动子，分别生成诱导型表面展示和分泌表达系统，命名为 rLc:pPG611-VP2-LTB（重组乳杆菌）和 rLc:pPG612-VP2-LTB。融合蛋白的表达通过 SDS-PAGE、Western blot 免疫荧光和电子显微镜进行验证。结果观察到，用 VP2-LTB 口服免疫的小鼠的 IgG 或 sIgA 水平高于接受 VP2 和阴性对照的小鼠，这表明存在显著的统计学差异。特别是，在本研究中，用 rLc:pPG612-VP2-LTB 免疫的小鼠的 IgG 或 sIgA 的滴度最高。总之，LTB 作为黏膜佐剂能够有效地促进表达 VP2 融合蛋白的乳杆菌诱导黏膜和系统免疫。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/fef365044d53/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/e6a44c0e52c6/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/00e7c31dfc21/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/f6d35b6dd26a/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/d2a28a17dca0/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/bd96ecc140fe/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/fd05b10f61f2/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/fef365044d53/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/e6a44c0e52c6/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/00e7c31dfc21/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/f6d35b6dd26a/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/d2a28a17dca0/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/bd96ecc140fe/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/fd05b10f61f2/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/837e/7112623/fef365044d53/gr7.jpg

相似文献

1
Comparison of the immune responses induced by oral immunization of mice with Lactobacillus casei-expressing porcine parvovirus VP2 and VP2 fused to Escherichia coli heat-labile enterotoxin B subunit protein.比较经口免疫表达猪细小病毒 VP2 的干酪乳杆菌和 VP2 融合大肠杆菌不耐热肠毒素 B 亚单位蛋白诱导小鼠免疫应答的效果。
Comp Immunol Microbiol Infect Dis. 2011 Jan;34(1):73-81. doi: 10.1016/j.cimid.2010.02.004. Epub 2010 Mar 11.
2
Recombinant porcine rotavirus VP4 and VP4-LTB expressed in Lactobacillus casei induced mucosal and systemic antibody responses in mice.重组猪轮状病毒 VP4 和 VP4-LTB 在干酪乳杆菌中的表达诱导小鼠黏膜和系统抗体应答。
BMC Microbiol. 2009 Dec 4;9:249. doi: 10.1186/1471-2180-9-249.
3
[Co-expression of CSFV T cell epitope E290 peptide and PPV VP2 protein in Lactobacillus casei and determination of specific antibodies in immunized mice].[猪瘟病毒T细胞表位E290肽与猪细小病毒VP2蛋白在干酪乳杆菌中的共表达及免疫小鼠体内特异性抗体的测定]
Wei Sheng Wu Xue Bao. 2007 Aug;47(4):667-72.
4
Construction of recombinant Lactobacillus casei efficiently surface displayed and secreted porcine parvovirus VP2 protein and comparison of the immune responses induced by oral immunization.重组干酪乳杆菌高效表面展示和分泌猪细小病毒VP2蛋白的构建及口服免疫诱导的免疫反应比较
Immunology. 2008 May;124(1):68-75. doi: 10.1111/j.1365-2567.2007.02738.x. Epub 2007 Nov 22.
5
[Escherichia coli heat-labile enterotoxin B subunit enhances the immune response against canine parvovirus VP2 in mice immunized by VP2 DNA vaccine].[大肠杆菌不耐热肠毒素B亚基增强VP2 DNA疫苗免疫小鼠对犬细小病毒VP2的免疫应答]
Wei Sheng Wu Xue Bao. 2011 Jan;51(1):91-7.
6
Oral Immunization with Expressing the Porcine Circovirus Type 2 Cap and LTB Induces Mucosal and Systemic Antibody Responses in Mice.猪圆环病毒 2 型 Cap 和 LTB 表达的口服免疫可诱导小鼠黏膜和系统抗体反应。
Viruses. 2021 Jul 5;13(7):1302. doi: 10.3390/v13071302.
7
Immunogenicity of recombinant Lactobacillus casei-expressing F4 (K88) fimbrial adhesin FaeG in conjunction with a heat-labile enterotoxin A (LTAK63) and heat-labile enterotoxin B (LTB) of enterotoxigenic Escherichia coli as an oral adjuvant in mice.表达F4（K88）菌毛黏附素FaeG的重组干酪乳杆菌与产肠毒素大肠杆菌的热不稳定肠毒素A（LTAK63）和热不稳定肠毒素B（LTB）联合作为口服佐剂在小鼠中的免疫原性。
J Appl Microbiol. 2017 Feb;122(2):506-515. doi: 10.1111/jam.13352. Epub 2016 Dec 12.
8
Induction of immune responses in mice after intragastric administration of Lactobacillus casei producing porcine parvovirus VP2 protein.经胃内给予表达猪细小病毒VP2蛋白的干酪乳杆菌后小鼠免疫反应的诱导
Appl Environ Microbiol. 2007 Nov;73(21):7041-7. doi: 10.1128/AEM.00436-07. Epub 2007 Sep 7.
9
[The surface display of porcine parvovirus VP2 protein in Lactobacillus casei].[猪细小病毒VP2蛋白在干酪乳杆菌中的表面展示]
Sheng Wu Gong Cheng Xue Bao. 2007 Mar;23(2):315-8.
10
[Enterotoxigenic Escherichia coli heat-labile enterotoxin B subunit can efficiently induce and enhance the mucosal immune response to enterovirus 71].产肠毒素大肠杆菌热不稳定肠毒素B亚基可有效诱导并增强针对肠道病毒71型的黏膜免疫反应
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2014 Dec;30(12):1233-7.

引用本文的文献

1
Oral Delivery of Expressing Full-Length S Protein via Alginate-Chitosan Capsules Induces Immune Protection Against PEDV Infection in Mice.通过藻酸盐-壳聚糖胶囊口服递送全长S蛋白可诱导小鼠对猪流行性腹泻病毒感染产生免疫保护。
Vaccines (Basel). 2025 Apr 17;13(4):421. doi: 10.3390/vaccines13040421.
2
Evaluation of the immunogenicity of auxotrophic with CRISPR-Cas9D10A system-mediated chromosomal editing to express porcine rotavirus capsid protein .评价 CRISPR-Cas9D10A 系统介导的染色体编辑表达猪轮状病毒衣壳蛋白的缺陷型 的免疫原性 。
Virulence. 2022 Dec;13(1):1315-1330. doi: 10.1080/21505594.2022.2107646.
3
Recombinant Lactobacillus acidophilus expressing S and S domains of porcine epidemic diarrhea virus could improve the humoral and mucosal immune levels in mice and sows inoculated orally.

本文引用的文献

1
Mucosal immunity in mice induced by orally administered transgenic rice.口服转基因水稻诱导小鼠的黏膜免疫
Vaccine. 2009 Mar 4;27(10):1596-600. doi: 10.1016/j.vaccine.2008.12.042. Epub 2009 Jan 13.
2
Construction of recombinant Lactobacillus casei efficiently surface displayed and secreted porcine parvovirus VP2 protein and comparison of the immune responses induced by oral immunization.重组干酪乳杆菌高效表面展示和分泌猪细小病毒VP2蛋白的构建及口服免疫诱导的免疫反应比较
Immunology. 2008 May;124(1):68-75. doi: 10.1111/j.1365-2567.2007.02738.x. Epub 2007 Nov 22.
3
A novel recombinant virus-like particle vaccine for prevention of porcine parvovirus-induced reproductive failure.
表达猪流行性腹泻病毒 S 和 S 结构域的重组嗜酸乳杆菌可改善经口接种的小鼠和母猪的体液和黏膜免疫水平。
Vet Microbiol. 2020 Sep;248:108827. doi: 10.1016/j.vetmic.2020.108827. Epub 2020 Aug 16.
4
Recovery of recombinant Mycobacterium tuberculosis antigens fused with cell wall-anchoring motif (LysM) from inclusion bodies using non-denaturing reagent (N-laurylsarcosine).使用非变性试剂（N-月桂酰肌氨酸）从包涵体中回收与细胞壁锚定基序（LysM）融合的重组结核分枝杆菌抗原。
BMC Biotechnol. 2019 May 14;19(1):27. doi: 10.1186/s12896-019-0522-x.
5
Targeted and Repetitive Chromosomal Integration Enables High-Level Heterologous Gene Expression in .靶向且重复的染色体整合使. 能够实现高水平的异源基因表达。
Appl Environ Microbiol. 2019 Apr 18;85(9). doi: 10.1128/AEM.00033-19. Print 2019 May 1.
6
Molecular cloning and transcriptional regulation of Indian peafowl (Pavo cristatus) IFN-α gene.印度孔雀（Pavo cristatus）IFN-α 基因的分子克隆与转录调控。
Cell Stress Chaperones. 2019 Mar;24(2):323-332. doi: 10.1007/s12192-018-00962-0. Epub 2019 Jan 30.
7
Immune responses induced by recombinant Lactobacillus plantarum expressing the spike protein derived from transmissible gastroenteritis virus in piglets.猪传染性胃肠炎病毒刺突蛋白在重组植物乳杆菌中诱导的免疫应答。
Appl Microbiol Biotechnol. 2018 Oct;102(19):8403-8417. doi: 10.1007/s00253-018-9205-0. Epub 2018 Jul 18.
8
Construction and characterization of thymidine auxotrophic (ΔthyA) recombinant Lactobacillus casei expressing bovine lactoferricin.表达牛乳铁蛋白肽的胸苷营养缺陷型（ΔthyA）重组干酪乳杆菌的构建与鉴定
BMC Vet Res. 2018 Jun 27;14(1):206. doi: 10.1186/s12917-018-1516-y.
9
Oral recombinant Lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus.口服重组乳杆菌疫苗靶向肠道微皱褶细胞和树突状细胞，用于递送猪流行性腹泻病毒的核心中和表位。
Microb Cell Fact. 2018 Feb 9;17(1):20. doi: 10.1186/s12934-018-0861-7.
10
A phase trial of the oral Lactobacillus casei vaccine polarizes Th2 cell immunity against transmissible gastroenteritis coronavirus infection.口服干酪乳杆菌疫苗的1期试验使针对传染性胃肠炎冠状病毒感染的Th2细胞免疫极化。
Appl Microbiol Biotechnol. 2016 Sep;100(17):7457-69. doi: 10.1007/s00253-016-7424-9. Epub 2016 Mar 28.
一种用于预防猪细小病毒引起的繁殖障碍的新型重组病毒样颗粒疫苗。
Vaccine. 2006 Jun 29;24(26):5481-90. doi: 10.1016/j.vaccine.2006.03.089. Epub 2006 Apr 18.
4
Mucosal immunization with surface-displayed severe acute respiratory syndrome coronavirus spike protein on Lactobacillus casei induces neutralizing antibodies in mice.用表面展示严重急性呼吸综合征冠状病毒刺突蛋白的干酪乳杆菌对小鼠进行黏膜免疫可诱导产生中和抗体。
J Virol. 2006 Apr;80(8):4079-87. doi: 10.1128/JVI.80.8.4079-4087.2006.
5
Real-time PCR protocol for the detection of porcine parvovirus in field samples.用于检测田间样本中猪细小病毒的实时荧光定量PCR方法
J Virol Methods. 2006 Jun;134(1-2):257-60. doi: 10.1016/j.jviromet.2006.01.004. Epub 2006 Feb 9.
6
Improved uptake of plant-derived LTB-linked proteins in carp gut and induction of specific humoral immune responses upon infeed delivery.鲤鱼肠道中植物源LTB连接蛋白的摄取改善及通过饲料投喂诱导特异性体液免疫反应。
Fish Shellfish Immunol. 2006 Sep;21(3):251-60. doi: 10.1016/j.fsi.2005.12.001. Epub 2006 Feb 7.
7
Intragastric administration of Lactobacillus casei expressing transmissible gastroentritis coronavirus spike glycoprotein induced specific antibody production.口服表达传染性胃肠炎冠状病毒刺突糖蛋白的干酪乳杆菌可诱导特异性抗体产生。
Vaccine. 2005 Feb 3;23(11):1335-42. doi: 10.1016/j.vaccine.2004.09.015.
8
Protective immunity of SpaA-antigen producing Lactococcus lactis against Erysipelothrix rhusiopathiae infection.产SpaA抗原的乳酸乳球菌对猪丹毒杆菌感染的保护性免疫
J Appl Microbiol. 2004;96(6):1347-53. doi: 10.1111/j.1365-2672.2004.02283.x.
9
Expression of the B subunit of the heat-labile enterotoxin of Escherichia coli in tobacco mosaic virus-infected Nicotiana benthamiana plants and its characterization as mucosal immunogen and adjuvant.大肠杆菌不耐热肠毒素B亚基在烟草花叶病毒感染的本氏烟草植株中的表达及其作为黏膜免疫原和佐剂的特性研究
J Immunol Methods. 2004 Apr;287(1-2):203-15. doi: 10.1016/j.jim.2004.02.001.
10
Mice immunization with live lactococci displaying a surface anchored HPV-16 E7 oncoprotein.用展示表面锚定的人乳头瘤病毒16型E7癌蛋白的活乳酸乳球菌对小鼠进行免疫接种。
FEMS Microbiol Lett. 2003 Dec 5;229(1):37-42. doi: 10.1016/S0378-1097(03)00778-X.