Li Jun-Min, Jin Ze-Xin
Institute of Ecology, Taizhou University, Linhai, Zhejiang 317000, PR China.
Genetica. 2008 Jul;133(3):227-34. doi: 10.1007/s10709-007-9204-z. Epub 2007 Sep 8.
Inter-simple sequence repeat (ISSR) markers were used to determine the genetic variation and genetic differentiation of nine populations of Emmenopterys henryi Oliv., an endangered plant endemic to China. Relatively low genetic diversity was detected at population level (the percentage of polymorphic loci P=22.56%, the number of alleles per locus A=1.183+/-0.045, the effective number of alleles per locus A(E)=1.007+/-0.345, Nei's gene diversity h=0.071+/-0.017, Shannon information index I=0.104+/-0.025). However, the genetic diversity at species level was relatively high (P=56.05%; A=1.561+/-0.498, A(E)=1.325+/-0.371, h=0.191+/-0.199, I=0.287+/-0.284). Analysis of molecular variance showed that most of the ISSR variation (68.03%) in E. henryi occurred among populations. The estimated Nm from F (ST )was 0.235. It indicated that the fragmentation and isolation of populations might result from specific evolutionary history and anthropogenic activity. Consequently, genetic drift might play an important role in determining the genetic structure of E. henryi. Conservation strategies for this endangered species are proposed based on the genetic data.
利用简单序列重复区间（ISSR）标记来测定中国特有的濒危植物香果树9个种群的遗传变异和遗传分化。在种群水平上检测到相对较低的遗传多样性（多态位点百分比P = 22.56%，每个位点的等位基因数A = 1.183±0.045，每个位点的有效等位基因数A(E)= 1.007±0.345，Nei氏基因多样性h = 0.071±0.017，香农信息指数I = 0.104±0.025）。然而，在物种水平上遗传多样性相对较高（P = 56.05%；A = 1.561±0.498，A(E)= 1.325±0.371，h = 0.191±0.199，I = 0.287±0.284）。分子方差分析表明，香果树的ISSR变异大部分（68.03%）发生在种群间。根据F(ST)估计的Nm为0.235。这表明种群的片段化和隔离可能是由特定的进化历史和人为活动导致的。因此，遗传漂变可能在决定香果树的遗传结构中起重要作用。基于遗传数据提出了针对这种濒危物种的保护策略。
