Department of Horticultural, Faculty of Agriculture, University of Ilam, Ilam, Iran.
Mol Biol Rep. 2013 Nov;40(11):6501-8. doi: 10.1007/s11033-013-2768-z. Epub 2013 Oct 6.
Fifty-seven genotypes from eight population of Satureja bachtiarica was evaluated using fifteen ISSR and eleven RAPD markers. DNA profiling using RAPD primers amplified 84 loci, among which 81 were polymorphic with an average of 7.36 polymorphic fragments per locus. Also, using RAPD markers maximum and minimum polymorphic bands observed for Semyrom (77.38 %) and Farsan (40.48 %) populations, respectively. Semyrom population recorded the highest unbiased expected heterozygosity (0.259) and Shannon's Indices (0.38). While, the lowest values of unbiased expected heterozygosity (0.172) and Shannon's Index (0.245) were recorded for Eghlid and Farsan populations, respectively. On the other hand, ISSR primers produced 136 bands, from which 134 were polymorphic with an average of 9.06 polymorphic fragments per primer (98.52 %). The ISSR markers evaluation revealed that maximum and minimum polymorphic bands observed for Semyrom (66.18 %) and Farsan (31.62 %), respectively. Shahrekorud population recorded the highest unbiased expected heterozygosity (0.211) and Shannon's Indices (0.301). While, the lowest value of unbiased expected heterozygosity (0.175) observed for Farsan and Yazd populations and the lowest Shannon's Index (0.191) recorded by Farsan population. The overall results of the study revealed that both ISSR and RAPD markers were effective for evaluation of genetic variation of S. bachtiarica.
采用 15 条 ISSR 和 11 条 RAPD 标记对来自 8 个居群的 57 个基因型的八倍体天蓝鼠尾草进行了评估。RAPD 引物的 DNA 指纹图谱扩增了 84 个位点，其中 81 个为多态性，每个位点平均有 7.36 个多态性片段。此外，使用 RAPD 标记，Semyrom（77.38%）和 Farsan（40.48%）居群的观察到的最大和最小多态性带分别为 77.38%和 40.48%。Semyrom 居群记录的无偏期望杂合度（0.259）和 Shannon 指数（0.38）最高。而，Eghlid 和 Farsan 居群的无偏期望杂合度（0.172）和 Shannon 指数（0.245）的最低值分别为 0.172 和 0.245。另一方面，ISSR 引物产生了 136 条带，其中 134 条为多态性，每个引物的平均多态性片段数为 9.06（98.52%）。ISSR 标记的评估结果表明，Semyrom（66.18%）和 Farsan（31.62%）的最大和最小多态性带分别为 66.18%和 31.62%。Shahrekorud 居群记录的无偏期望杂合度（0.211）和 Shannon 指数（0.301）最高。而，Farsan 和 Yazd 居群的无偏期望杂合度（0.175）和 Farsan 居群的 Shannon 指数（0.191）的最低值。总的来说，研究结果表明，ISSR 和 RAPD 标记均能有效评价八倍体天蓝鼠尾草的遗传变异。
