Zhang Xiaoqiao, Mei Yuanwu, Liu Chuanyu, Yu Shanchun
Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2007 Aug;27(4):415-8. doi: 10.1007/s11596-007-0416-3.
The effect of transcranial magnetic stimulation (TMS) on the neurological functional recovery and expression of c-Fos and brain-derived neurotrophic factor (BDNF) of the cerebral cortex in rats with cerebral infarction was investigated. Cerebral infarction models were established by using left middle cerebral artery occlusion (MCAO) and were randomly divided into a model group (n=40) and a TMS group (n=40). TMS treatment (2 times per day, 30 pulses per time) with a frequency of 0.5 Hz and magnetic field intensity of 1.33 Tesla was carried out in TMS group after MCAO. Modified neurological severity score (NSS) were recorded before and 1, 7, 14, 21, and 28 day(s) after MCAO. The expression of c-Fos and BDNF was immunohistochemically detected 1, 7, 14, 21, and 28 day(s) after infarction respectively. Our results showed that a significant recovery of NSS (P<0.05) was found in animals treated by TMS on day 7, 14, 21, and 28 as compared with the animals in the model group. The positive expression of c-Fos and BDNF was detected in the cortex surrounding the infarction areas, while the expression of c-Fos and BDNF increased significantly in TMS treatment group in comparison with those in model group 7, 14, 21, and 28 days (P<0.05) and 7, 14, 21 days (P<0.01) after infarction, respectively. It is concluded that TMS has therapeutic effect on cerebral infarction and this may have something to do with TMS's ability to promote the expression of c-Fos and BDNF of the cerebral cortex in rats with cerebral infarction.
探讨经颅磁刺激（TMS）对脑梗死大鼠神经功能恢复及大脑皮质c-Fos和脑源性神经营养因子（BDNF）表达的影响。采用左侧大脑中动脉闭塞（MCAO）法建立脑梗死模型，将大鼠随机分为模型组（n=40）和TMS组（n=40）。MCAO术后对TMS组进行TMS治疗（每天2次，每次30个脉冲），频率为0.5Hz，磁场强度为1.33特斯拉。分别在MCAO术前及术后1、7、14、21和28天记录改良神经功能缺损评分（NSS）。分别在梗死术后1、7、14、21和28天采用免疫组织化学法检测c-Fos和BDNF的表达。结果显示，与模型组相比，TMS治疗组大鼠在术后7、14、21和28天NSS显著恢复（P<0.05）。梗死灶周围皮质检测到c-Fos和BDNF的阳性表达，与模型组相比，TMS治疗组梗死术后7、14、21和28天c-Fos和BDNF的表达显著增加（P<0.05），术后7、14、21天BDNF表达显著增加（P<0.01）。结论：TMS对脑梗死具有治疗作用，这可能与TMS促进脑梗死大鼠大脑皮质c-Fos和BDNF表达的能力有关。
