Polanska M A, Yasuda A, Harzsch S
Institute of Infectious Diseases, Medical University of Warsaw, Warsaw, 02-106, Poland.
Cell Tissue Res. 2007 Nov;330(2):331-44. doi: 10.1007/s00441-007-0473-8. Epub 2007 Sep 9.
Crustacean-SIFamide (GYRKPPFNGSIFamide) is a novel neuropeptide that was recently isolated from crayfish nervous tissue. We mapped the localisation of this peptide in the median brain and eyestalk neuropils of the marbled crayfish (Marmorkrebs), a parthenogenetic crustacean. Our experiments showed that crustacean-SIFamide is strongly expressed in all major compartments of the crayfish brain, including all three optic neuropils, the lateral protocerebrum with the hemiellipsoid body, and the medial protocerebrum with the central complex. These findings imply a role of this peptide in visual processing already at the level of the lamina but also at the level of the deeper relay stations. Immunolabelling is particularly strong in the accessory lobes and the deutocerebral olfactory lobes that receive a chemosensory input from the first antennae. Most cells of the olfactory globular tract, a projection neuron pathway that links deuto- and protocerebrum, are labelled. This pathway plays a central role in conveying tactile and olfactory stimuli to the lateral protocerebrum, where this input converges with optic information. Weak labelling is also present in the tritocerebrum that is associated with the mechanosensory second antennae. Taken together, we suggest an important role of crustacean-SIFamidergic neurons in processing high-order, multimodal input in the crayfish brain.
甲壳类动物SIF酰胺（GYRKPPFNGSIF酰胺）是一种新型神经肽，最近从小龙虾神经组织中分离出来。我们绘制了这种肽在大理石纹螯虾（Marmorkrebs）的中脑和眼柄神经纤维网中的定位图，大理石纹螯虾是一种孤雌生殖的甲壳类动物。我们的实验表明，甲壳类动物SIF酰胺在小龙虾大脑的所有主要区域都有强烈表达，包括所有三个视神经纤维网、带有半椭球体的外侧原脑和带有中央复合体的内侧原脑。这些发现表明，这种肽在视觉处理中不仅在神经层水平，而且在更深层中继站水平都发挥作用。免疫标记在副叶和接受第一触角化学感觉输入的中脑嗅觉叶中尤为强烈。嗅觉球状束的大多数细胞都被标记，嗅觉球状束是一条连接中脑和原脑的投射神经元通路。这条通路在将触觉和嗅觉刺激传递到外侧原脑方面起着核心作用，在外侧原脑这种输入与视觉信息汇聚。与机械感觉第二触角相关的后脑也有较弱标记。综上所述，我们认为甲壳类动物SIF酰胺能神经元在小龙虾大脑处理高阶多模态输入中起重要作用。
