Science. 1962 Nov 9;138(3541):690-1. doi: 10.1126/science.138.3541.690.
In laboratory inoculation experiments, Rosen rye seedlings were more susceptible, Genesee wheat seedlings were less susceptible, and Trail barley seedlings were not susceptible to Claviceps purpurea. Guttation fluids from these plants were used as media for the growth of germinating spores of C. purpurea. Growth experiments consisted of spores which were germinated in thin agar films on microscope slides. Germ tube growth was determined by counting the tubes that crossed the perimeter of a square in the microscope field, counting the conidia in the square, and computing the number of crosses per conidium. Rye guttation fluid produced the most growth and barley fluid the least. Thus the degree of susceptibility is correlated with the amount of growth of the parasite in vitro.
在实验室接种实验中，罗森黑麦幼苗更易感染，吉内斯小麦幼苗较不易感染，而垂穗大麦幼苗则不易感染麦角菌。这些植物的溢泌液被用作麦角菌萌发孢子生长的培养基。生长实验包括在显微镜载玻片上的薄琼脂膜中萌发的孢子。通过数穿过显微镜视野中方格周长的管数、数方格中的分生孢子并计算每个分生孢子的交叉数来确定芽管生长。黑麦溢泌液产生的生长最多，大麦溢泌液产生的生长最少。因此，体外寄生虫的生长程度与易感性程度相关。
