Tsan M F, Newman B, McIntyre P A
Br J Haematol. 1976 Jun;33(2):189-204. doi: 10.1111/j.1365-2141.1976.tb03530.x.
The role of surface sulphydryl (-SH) groups of human polymorphonuclear leucocytes on phagocytosis-associated oxidative metabolic changes was studied. p-Chloromercurybenzene sulphonic acid, a surface -SH group inhibitor, had no effect on phagocytosis, superoxide production during phagocytosis, or killing of S. aureus by the leucocytes, while it inhibited phagocytosis-associated stimulation of hexose monophosphate pathway activity and H2O2 production. In contrast, N-ethylmaleimide, which inhibits both intracellular and surface -SH groups, inhibited phagocytosis-associated oxidative metabolic changes. p-Chloromercurybenzene sulphonic acid also inhibited the stimulation of hexose monophosphate pathway activity by exogenous H2O2, suggesting a regulatory role of plasma membrane on the pathway's activity in human polymorphonuclear leucocytes. The results also suggest that: (I) superoxide production is the primary event of oxidative metabolic changes during phagocytosis, (2) superoxide plays an important role in the killing of S. aureus, and (3) the hexose monophosphate pathway plays a primary role in producing NADPH for H2O2 production from superoxide.
研究了人类多形核白细胞表面巯基（-SH）基团在吞噬作用相关氧化代谢变化中的作用。对氯汞苯磺酸，一种表面-SH基团抑制剂，对吞噬作用、吞噬过程中的超氧化物产生或白细胞对金黄色葡萄球菌的杀伤均无影响，然而它却抑制了与吞噬作用相关的己糖磷酸途径活性的刺激以及过氧化氢的产生。相反，N-乙基马来酰亚胺，它既能抑制细胞内也能抑制表面的-SH基团，抑制了与吞噬作用相关的氧化代谢变化。对氯汞苯磺酸也抑制了外源性过氧化氢对己糖磷酸途径活性的刺激，这表明质膜对人类多形核白细胞中该途径的活性具有调节作用。结果还表明：（1）超氧化物产生是吞噬作用期间氧化代谢变化的主要事件，（2）超氧化物在金黄色葡萄球菌的杀伤中起重要作用，以及（3）己糖磷酸途径在为超氧化物产生过氧化氢而生成烟酰胺腺嘌呤二核苷酸磷酸（NADPH）过程中起主要作用。
