Tsan M F, McIntyre P A
Br J Haematol. 1975 Oct;31(2):193-208. doi: 10.1111/j.1365-2141.1975.tb00850.x.
The effect of propylthiouracil on glucose metabolism in human polymorphonuclear leucocytes was studied. At a therapeutically achievable concentration (0.1 mM), propylthiouracil stimulated hexose monophosphate shunt activity in normal leucocytes during phagocytosis but not in resting cells. However, in the presence of hydrogen peroxide it stimulated hexose monophosphate shunt activity in resting cells, and in the soluble fraction when reduced glutathione and reduced nictotinamide adenine dinucleotide phosphate (NADPH) were also present. Propylthiouracil had nor effect on glucose-1-C oxidation in either phagocytosing or resting leucocytes obtained from two male patients with chronic granulomatous disease. Stimulation of the hexose monophosphate shunt activity in normal leucocytes during phagocytosis also was demonstrated with methimazole, thiouracil and thiourea, but not with adenine, uracil or urea. There was an apparent minimal common structure requirement in thriourea. Propylthiouracil had no effect on phagocytosis, formate oxidation, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase or catalase activities. Thus, the stimulation of the hexose monophosphate shunt activity by propylthiouracil is dependent on hydrogen peroxide and is best explained by its stimulation or participation in the glutathione cycle.
研究了丙硫氧嘧啶对人多形核白细胞葡萄糖代谢的影响。在治疗可达到的浓度（0.1 mM）下，丙硫氧嘧啶在吞噬作用期间刺激正常白细胞中的磷酸己糖旁路活性，但对静息细胞无此作用。然而，在过氧化氢存在的情况下，它刺激静息细胞中的磷酸己糖旁路活性，并且在同时存在还原型谷胱甘肽和还原型烟酰胺腺嘌呤二核苷酸磷酸（NADPH）时刺激可溶性部分中的活性。丙硫氧嘧啶对从两名慢性肉芽肿病男性患者获得的吞噬或静息白细胞中的葡萄糖-1-C氧化均无影响。甲巯咪唑、硫氧嘧啶和硫脲也证明了在吞噬作用期间对正常白细胞中磷酸己糖旁路活性的刺激，但腺嘌呤、尿嘧啶或尿素则无此作用。硫脲类药物存在明显的最小共同结构要求。丙硫氧嘧啶对吞噬作用、甲酸氧化、葡萄糖-6-磷酸脱氢酶、6-磷酸葡萄糖酸脱氢酶或过氧化氢酶活性均无影响。因此，丙硫氧嘧啶对磷酸己糖旁路活性的刺激依赖于过氧化氢，并且最好用其对谷胱甘肽循环的刺激或参与来解释。
