Baehner R L, Murrmann S K, Davis J, Johnston R B
J Clin Invest. 1975 Sep;56(3):571-6. doi: 10.1172/JCI108126.
The contribution of hydrogen peroxide (H2O2) and one of its unstable intermediates, superoxide anion (O2), to the oxidative reactions that occur in phagocytizing leukocytes was explored by depleting these cells of O2. This was accomplished by allowing them to phagocytize latex particles coated with superoxide dismutase (SOD), which catalyzes the generation of H2O2 from O2. Although the rate and extent of phagocytosis of latex coated with bovine serum albumin was similar to latex coated with SOD, the rate of oxygen consumption, [14C]formate oxidation, [1-14C]glucose oxidation, and iodination of zymosan particles was significantly enhanced by SOD. In contrast, the rate and extent of reduction of nitroblue tetrazolium (NBT) was diminished by 60%. These studies indicate that the majority of NBT reduction by leukocytes is due to O2, whereas stimulation of the hexose monophosphate shunt and iodination of ingested particles requires H2O2 generated from the increased reduction of oxygen by phagocytizing leukocytes.
通过耗尽吞噬白细胞中的超氧阴离子（O₂），研究了过氧化氢（H₂O₂）及其不稳定中间体之一超氧阴离子（O₂）对吞噬白细胞中发生的氧化反应的贡献。这是通过让它们吞噬包被超氧化物歧化酶（SOD）的乳胶颗粒来实现的（SOD催化O₂生成H₂O₂）。虽然包被牛血清白蛋白的乳胶的吞噬速率和程度与包被SOD的乳胶相似，但SOD显著提高了耗氧速率、[¹⁴C]甲酸氧化速率、[1-¹⁴C]葡萄糖氧化速率以及酵母聚糖颗粒的碘化速率。相比之下，硝基蓝四氮唑（NBT）还原的速率和程度降低了60%。这些研究表明，白细胞对NBT的还原大部分是由于O₂，而己糖磷酸旁路的刺激和摄入颗粒的碘化则需要吞噬白细胞增加对氧的还原所产生的H₂O₂。
