Díaz-Laviada I, Ainaga J, Portolés M T, Carrascosa J L, Muncio A M, Pagani R
Department of Biochemistry and Molecular Biology, Faculty of Chemistry, Universidad Complutense, Madrid, Spain.
Histochem J. 1991 May;23(5):221-8. doi: 10.1007/BF01462244.
In this study, the uptake and localization of an Escherichia coli lipopolysaccharide, and the temperature effects on these processes, were studied in rat cultured hepatocytes using a binding assay and an immunocolloidal gold technique. The lipopolysaccharide was found to bind to the cell membrane and microvilli after short incubation times, at both 4 degrees C and 37 degrees C. This was followed by a dispersed localization into the cytoplasm, reaching mitochondria. The uptake was found not to be receptor-mediated. A decrease of temperature, delays, but does not prevent, the lipopolysaccharide internalization.
在本研究中，使用结合测定法和免疫胶体金技术，在大鼠培养肝细胞中研究了大肠杆菌脂多糖的摄取和定位，以及温度对这些过程的影响。发现脂多糖在4℃和37℃下短时间孵育后均与细胞膜和微绒毛结合。随后分散定位到细胞质中，到达线粒体。发现摄取不是受体介导的。温度降低会延迟但不会阻止脂多糖的内化。
