Kimber I, Cumberbatch M, Coleman J W
Immunology Group, ICI Central Toxicology Laboratory, Macclesfield, Cheshire, UK.
J Appl Toxicol. 1991 Oct;11(5):339-42. doi: 10.1002/jat.2550110507.
We report that challenge of previously contact-sensitized mice results in a significant increase in the concentration of serum histamine. In an attempt to determine whether this phenomenon might form the basis of an alternative method for the evaluation of elicitation reactions in experimental contact sensitivity, we have compared challenge-induced increases in ear thickness with elevations in serum histamine. Challenge of sensitized mice revealed that both ear thickness changes and increases in the serum level of histamine were dependent upon the concentration of oxazolone used for sensitization. The kinetics of changes in serum histamine concentration were found to be biphasic, with a small increase measurable 2 h following challenge and the maximal response at 24 or 48 h. In contrast, increases in ear thickness were monophasic, although maximum responses were also observed at 24 h. It is concluded that, although they do not exactly parallel increases in ear thickness, changes in histamine concentration may provide a useful serological correlate of the challenge reaction in contact sensitivity.
我们报告称，对先前已接触致敏的小鼠进行激发会导致血清组胺浓度显著升高。为了确定这一现象是否可能构成评估实验性接触敏感性激发反应的另一种方法的基础，我们将激发诱导的耳部厚度增加与血清组胺升高进行了比较。对致敏小鼠的激发显示，耳部厚度变化和血清组胺水平升高均取决于用于致敏的恶唑酮浓度。发现血清组胺浓度变化的动力学呈双相性，激发后2小时可测得小幅升高，最大反应出现在24或48小时。相比之下，耳部厚度增加呈单相性，尽管最大反应也出现在24小时。结论是，尽管组胺浓度变化与耳部厚度增加并不完全平行，但它们可能为接触敏感性激发反应提供有用的血清学关联指标。
