Miller K, Maisey J, Malkovský M
Int Arch Allergy Appl Immunol. 1984;75(2):120-5. doi: 10.1159/000233601.
Various methods have been used to enhance the ability of laboratory animals to develop contact sensitivity to so-called weak sensitizers. The present studies have examined whether diets supplemented with vitamin A acetate (VAA) could enhance induction and elicitation of contact sensitivity to oxazolone in the mouse. Application of 0.3% oxazolone to VAA-fed mice resulted in increased DNA synthesis in the draining lymph nodes and increased ear thickness after challenge, in comparison with mice on a stock (standard) diet. Moreover, VAA-fed (but not control) mice were sensitized to 0.03% oxazolone as shown by DNA synthesis, increased arrival of syngeneic 51Cr-labelled lymphocytes in the draining lymph nodes, and ear swelling after challenge. These findings demonstrate that vitamin A enhances delayed hypersensitivity responses, and point to a role for vitamin A in developing an animal model capable of detecting weak contact sensitizers after topical application.
人们已经采用了各种方法来提高实验动物对所谓弱致敏剂产生接触敏感性的能力。目前的研究探讨了补充醋酸维生素A（VAA）的饮食是否能增强小鼠对恶唑酮的接触敏感性的诱导和激发。与食用常规（标准）饮食的小鼠相比，给食用VAA的小鼠涂抹0.3%恶唑酮后，引流淋巴结中的DNA合成增加，激发后耳部厚度增加。此外，如DNA合成、引流淋巴结中同基因51Cr标记淋巴细胞的到达增加以及激发后耳部肿胀所示，食用VAA的（而非对照）小鼠对0.03%恶唑酮产生了致敏。这些发现表明维生素A增强了迟发型超敏反应，并指出维生素A在建立一种能够在局部应用后检测弱接触致敏剂的动物模型中发挥作用。
