Watanabe K, Shirabe M, Nakashima Y, Kakita Y
Microbiology Laboratory, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
J Gen Microbiol. 1991 Nov;137(11):2601-3. doi: 10.1099/00221287-137-11-2601.
The process of genome DNA injection, after adsorption, by phage PL-1 into host cells of Lactobacillus casei was monitored by using the electron microscope. Injection of DNA was inhibited by the protein-synthesis inhibitors chloramphenicol and erythromycin at concentrations where the colony-forming ability of cells not infected by phage was unaffected. The results suggest that protein synthesis may be involved in some way in the process of genome injection.
通过电子显微镜监测噬菌体PL-1吸附后将基因组DNA注入干酪乳杆菌宿主细胞的过程。在不影响未被噬菌体感染细胞的集落形成能力的浓度下,蛋白质合成抑制剂氯霉素和红霉素抑制了DNA的注入。结果表明,蛋白质合成可能以某种方式参与基因组注入过程。