Watanabe K, Fukuzaki T, Shirabe M, Nakashima Y, Murata K, Kuroiwa A
Faculty of Pharmaceutical Sciences, Fukuoka University.
Microbiol Immunol. 1990;34(5):471-5. doi: 10.1111/j.1348-0421.1990.tb01030.x.
Ultrathin sections of the cells of Lactobacillus casei infected with or without PL-1 phages were observed by the rapid-freezing and substitution-fixation method. Phage-head-like particles were first observed in the nuclear region. The region was seen more widely dispersed in the cytoplasm than that observed by the conventional chemical fixation method. The features of cells just broken open by the infected phages were observed by the sedimentation method devised by us. The bursting occurred in more than one place in the cells with liberation of progeny phages.
采用快速冷冻和置换固定法观察了感染或未感染PL - 1噬菌体的干酪乳杆菌细胞的超薄切片。首先在核区域观察到噬菌体头部样颗粒。与传统化学固定法观察到的相比，该区域在细胞质中分布更广泛。通过我们设计的沉降法观察了被感染噬菌体刚裂解的细胞特征。细胞内不止一处发生裂解，同时释放出子代噬菌体。
