Samtani J R, Wagle S U, Irani S F, Kshirsagar N A
Department of Pharmacology, Microbiology and Paediatrics, Seth G. S. Medical College, Parel, Bombay, Maharashtra.
J Postgrad Med. 1991 Jul;37(3):160-2.
Polymorphonuclear neutrophils play an important role in host defense mechanism. To assess PMN function in neonate a micromethod using only a few drops of blood was standardised. PMN adherent to coverslip were incubated with bacteria and using a differential staining with Acridine Orange (AO) phagocytic and bactericidal capacity were estimated.