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[猫、狐、大鼠和人类胎儿腺垂体中的促肾上腺皮质激素细胞和促黑素细胞:诱导荧光、细胞免疫技术和铅苏木精研究]

[Corticotrope and melanotrope cells in cat, fox, rat and human fetus adenohypophysis: studies with induced fluorescence, cytoimmunologic technics and lead hematoxyline].

作者信息

Bugnon C, Lenys D, Bloch B, Fellmann D

出版信息

C R Seances Soc Biol Fil. 1975;169(5):1271-6.

PMID:178416
Abstract

Formaldehyde-induced fluorescence, acid-catalyzed or not, methods, and immunocytology with anti-ACTH (1-24), anti-ACTH(17-39), ANTI-BETA-LPH immunserums were applied on the same preparations of cat, fox, rat and human foetus pituitaries. The superpositions of results showed that the pars intermedia and pars distalis corticomelano-lipotrophic cells of fox and cat pituitary, those of human foetal pituitary, and the purely corticotrophics cells of the rat pars distalis contained a fluorogenic probably granular compound. Moreover, the granules of the same cell types were electively revealed on our lymphilized material by plombic hematoxylin. Only the anti-beta-LPH and/or anti-beta-MSH fixing celpls exhibited hypercyanophilic, PAS-positive and bleu alcian-positive caracteristics.

摘要

甲醛诱导荧光法,无论是否经过酸催化,以及使用抗促肾上腺皮质激素(1-24)、抗促肾上腺皮质激素(17-39)、抗β-促脂素免疫血清的免疫细胞学法,都应用于猫、狐狸、大鼠和人类胎儿垂体的相同制剂上。结果叠加显示,狐狸和猫垂体的中间部和远侧部皮质黑素促脂细胞、人类胎儿垂体的这些细胞以及大鼠远侧部的纯促肾上腺皮质激素细胞含有一种可能呈颗粒状的荧光化合物。此外,通过铅苏木精在我们的冻干材料上选择性地显示出相同细胞类型的颗粒。只有抗β-促脂素和/或抗β-促黑素固定细胞表现出嗜深青色、PAS阳性和阿尔辛蓝阳性特征。

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