Titok M A, Olekhnovich I N, Evtushenkov A N
Mol Gen Mikrobiol Virusol. 1991 Aug(8):20-2.
The rep-region of a broad host range plasmid pM3 (IncP-9) has been cloned by connecting the 7.2 kb PstI fragment of the plasmid with the 1.4 kb PstI fragment of the pUC4K vector plasmid. The latter includes the gene for kanamycin resistance and is unable to replicate by itself. The obtained minireplicon designated pMT2 contains rep-genes permitting its inheritance in the different gram-negative cells and a mob-site coding for incompatibility and temperature instability characteristic of the parent plasmid pM3.
通过将广宿主范围质粒pM3（IncP-9）的7.2 kb PstI片段与pUC4K载体质粒的1.4 kb PstI片段连接，克隆了该质粒的复制区。后者包含卡那霉素抗性基因，自身无法复制。获得的命名为pMT2的微型复制子包含允许其在不同革兰氏阴性细胞中遗传的复制基因，以及一个编码亲本质粒pM3不相容性和温度不稳定性特征的mob位点。
