Zakharenko V I, Gorelov V N, Seliutina D F, Kulakov Iu K, Nenashev A V, Skavronskaia A G
Mol Gen Mikrobiol Virusol. 1990 Jan(1):22-6.
The birepliconed plasmid pOV13 possesses all the properties of a vector for DNA cloning in a broad host range of bacterial cells. pOV13 is transfered by transformation and stably inherited by Escherichia coli, Brucella, Pseudomonas cells determining the resistance to streptomycin, tetrocycline and kanamycin in these bacteria. The plasmid pOV13 is a multicopy plasmid optimal in replication capacity (23kb). The plasmid carries single sites for some restriction endonucleases that are used for DNA cloning, including some restriction sites in antibiotic resistance genes. The examples of DNA cloning with the selection of recombinant clones by the insertional inactivation of kanamycin or tetracycline resistance and expression of the cloned DNAs are presented.
双复制子质粒pOV13具有在广泛宿主范围的细菌细胞中作为DNA克隆载体的所有特性。pOV13通过转化进行转移，并由大肠杆菌、布鲁氏菌、假单胞菌细胞稳定遗传，这些细胞决定了这些细菌对链霉素、四环素和卡那霉素的抗性。质粒pOV13是一种复制能力最佳（23kb）的多拷贝质粒。该质粒带有一些用于DNA克隆的限制性内切酶的单一位点，包括抗生素抗性基因中的一些限制性位点。文中给出了通过卡那霉素或四环素抗性的插入失活选择重组克隆以及克隆DNA表达来进行DNA克隆的实例。
