van Delft J L, van Haeringen N J, Bodelier V M, Barthen E R, Oosterhuis J A
Department of Ophthalmology, Leiden Medical Center, The Netherlands.
Ophthalmic Res. 1991;23(5):240-5. doi: 10.1159/000267119.
Rabbit eyes were enucleated and frozen in liquid nitrogen. The vitreous was removed and analyzed for prostaglandin E2 (PGE2). The mean level (+/- SEM) of PGE2 in the anterior as well as in the posterior vitreous was 0.09 +/- 0.016 ng/ml (n = 12 rabbits). Animals pretreated with indomethacin 15 min before death, in order to prevent the formation of prostaglandin during enucleation, showed in both the anterior and posterior vitreous mean PGE2 levels of 0.04 +/- 0.008 ng/ml (n = 12) which were significantly lower. Ocular trauma such as paracentesis of the anterior chamber, indentation of the sclera or laser photocoagulation in the fundus 1 h before death of the animals did not increase the concentration of PGE2 in the vitreous humor. There is no evidence either for local release of PGE2 from tissues in the fundus of the eye or for diffusion of PGE2 from the anterior chamber into the vitreous humor.
将兔眼摘除并置于液氮中冷冻。取出玻璃体并分析其中的前列腺素E2（PGE2）。前玻璃体和后玻璃体中PGE2的平均水平（±SEM）为0.09±0.016 ng/ml（n = 12只兔子）。在处死前15分钟用吲哚美辛预处理动物，以防止摘除眼球过程中前列腺素的形成，结果显示前玻璃体和后玻璃体中的平均PGE2水平为0.04±0.008 ng/ml（n = 12），显著较低。在动物死亡前1小时进行的眼部创伤，如前房穿刺、巩膜压陷或眼底激光光凝，并未增加玻璃体液中PGE2的浓度。没有证据表明眼内组织局部释放PGE2，也没有证据表明PGE2从前房扩散到玻璃体液中。
