Liang C, Peyman G A, Serracarbassa P, Calixto N, Chow A A, Rao P
LSU Eye Center, Louisiana State University Medical Center School of Medicine, New Orleans, USA.
Int Ophthalmol. 1998;22(1):13-8. doi: 10.1023/a:1006016809070.
To evaluate ocular tolerance of methylated collagen gel injected intravitreally and into the anterior chamber.
Methylated collagen (type I/III) was tested in New Zealand white rabbits. Vitreous cavity: After pars plana vitrectomy, methylated collagen gel was injected intravitreally. The eyes were examined clinically; electroretinogram recordings were made before and after surgery. Vitreous samples were taken for immunological analysis for the presence of the injected collagen. The rabbits were sacrificed 6 months after surgery; the retina was evaluated by light microscopy. Anterior chamber: In another group of rabbits, methylated collagen gel (0.2 ml, 0.1 ml, or 0.05 ml) was injected into the anterior chamber after paracentesis. The eyes were examined with a slit lamp; intraocular pressure was measured postinjection. The rabbits were sacrificed after 4 months; the corneas were evaluated histologically.
Vitreous cavity: The fundus view was clear for 6 months after intravitreal injection. Scotopic and photopic electroretinograms were normal in 6/7 eyes; one eye experienced a mild decrease one month postoperatively. No abnormal changes were found in the retinal histology. Anterior chamber: Some corneas were hazy and edematous around the injection site for one week. The injected collagen appeared in bundles, patches, and little pieces in the anterior chamber with precipitates on the corneal endothelium, pupillary margin, and the anterior capsule of the lens. The collagen diminished gradually, without causing permanent opacity. Histologically, the corneal endothelium in the eye which received 0.2 ml collagen showed a mild distention of the mitochondriae and vesicle formation between endothelial cells under transmission electron microscope.
Methylated collagen gel was tolerated by the eye after intravitreal injection. Localized temporary clinical and mild ultrastructural corneal changes were observed after anterior chamber injection.
评估玻璃体内和前房内注射甲基化胶原凝胶的眼耐受性。
在新西兰白兔中测试甲基化胶原（I/III型）。玻璃体腔：在经扁平部玻璃体切除术后，将甲基化胶原凝胶注射到玻璃体内。对眼睛进行临床检查；在手术前后进行视网膜电图记录。采集玻璃体样本进行免疫分析，以检测注射的胶原的存在。术后6个月处死兔子；通过光学显微镜评估视网膜。前房：在另一组兔子中，在穿刺后将甲基化胶原凝胶（0.2 ml、0.1 ml或0.05 ml）注射到前房内。用裂隙灯检查眼睛；注射后测量眼压。4个月后处死兔子；对角膜进行组织学评估。
玻璃体腔：玻璃体内注射后6个月眼底视图清晰。7只眼中6只的暗视和明视视网膜电图正常；1只眼在术后1个月出现轻度下降。视网膜组织学未发现异常变化。前房：注射部位周围的一些角膜在1周内模糊且水肿。注射的胶原在前房内呈束状、片状和小片状出现，在角膜内皮、瞳孔边缘和晶状体前囊上有沉淀物。胶原逐渐减少，未引起永久性混浊。组织学上，接受0.2 ml胶原的眼中，在透射电子显微镜下，角膜内皮显示线粒体轻度扩张，内皮细胞之间有小泡形成。
玻璃体内注射后甲基化胶原凝胶可被眼睛耐受。前房注射后观察到局部短暂的临床和轻度超微结构角膜变化。
