Vianna W L, Bruno D G, Namindome A, de Campos Rosseto A C, Barnabé R C, de Sant' Anna Moretti A
Laboratório de Pesquisa em Suínos, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, Brazil.
Reprod Domest Anim. 2007 Oct;42(5):466-70. doi: 10.1111/j.1439-0531.2006.00808.x.
The correct measurement of spermatic concentration in boar semen is one of the basic tasks routinely performed at the Swine Artificial Insemination Center (CIAS). This article explains two experiments whose objectives were: Experiment 1: This trial had two objectives: (1) to evaluate the precision of the spermdensimeter (SPM) with relation to the Neubauer count chamber (CN) which is considered the standard technique in the field and (2) to correct the scale on the SPM table. Experiment 2: This trial also had two objectives: (1) to evaluate the time and repetitions required in the SPM and (2) to compare the SPM adjusted table produced in Expt 1 with the SPM standard table. The methods in Expt 1 were used to measure total spermatic concentration in 141 boar ejaculates. The average spermatic concentrations (10(6) spermatozoids/ml) and average dose number were 229.1 and 22.6 for CN, and 283.6 and 28.0 for SPM. These results indicate that the SPM has a tendency to overestimate spermatic concentration with relation to the CN. The SPM produced a smaller coefficient of variation and needed lesser examination time than the CN (p < 0.05). The SPM adjusted table created in Expt 1 produced similar results as the SPM and CN standard tables (2.96 billion live spermatozoids/dose vs 2.36 billion live spermatozoids/dose). Incorporation and evaluation of supplemental data from other CIAS regarding the use of the SPM adjusted table in the field entitled 'Adjusted Table for Karras Spermdensimeter LPS-FMVZ-USP' would be valuable to prove its efficiency.
公猪精液中精子浓度的准确测量是猪人工授精中心（CIAS）日常进行的基本任务之一。本文介绍了两个实验，其目的如下：实验1：本试验有两个目的：（1）评估精子密度计（SPM）相对于纽鲍尔计数板（CN）的精度，后者被认为是该领域的标准技术；（2）校正SPM表上的刻度。实验2：本试验也有两个目的：（1）评估SPM所需的时间和重复次数；（2）将实验1中产生的SPM调整表与SPM标准表进行比较。实验1中的方法用于测量141份公猪精液中的总精子浓度。对于CN，平均精子浓度（10⁶个精子/ml）和平均剂量数分别为229.1和22.6；对于SPM，分别为283.6和28.0。这些结果表明，相对于CN，SPM有高估精子浓度的趋势。与CN相比，SPM产生的变异系数更小，检查时间更短（p < 0.05）。实验1中创建的SPM调整表产生的结果与SPM和CN标准表相似（29.6亿个活精子/剂量对23.6亿个活精子/剂量）。纳入并评估来自其他CIAS关于在“Karras精子密度计LPS-FMVZ-USP调整表”领域使用SPM调整表的补充数据，对于证明其有效性将是有价值的。
