Hansen C, Vermeiden T, Vermeiden J P W, Simmet C, Day B C, Feitsma H
Danish Pig Production, Danish Meat Association, Axeltorv 3, DK-1609 Copenhagen V, Denmark.
Theriogenology. 2006 Dec;66(9):2188-94. doi: 10.1016/j.theriogenology.2006.05.020. Epub 2006 Aug 21.
Current research aims at reducing the number of sperm per insemination dose thereby making measurement of sperm concentration in raw semen and the production of uniform insemination doses much more crucial. The present study evaluated the determination of sperm concentration using FACSCount AF System (FACS), Improved Neubauer hemocytometer (HEMO), Corning 254 photometer (Photo C254), SpermVision CASA System (SpermVision), UltiMate CASA System (UltiMate) and NucleoCounter SP-100 (SP-100). The instruments were evaluated with respect to repeatability and to establishing the regression curve towards both HEMO and FACS. Repeatability for the instruments was 2.7, 7.1, 10.4, 8.1, 5.4 and 3.1% for FACS, HEMO, Photo C254, SpermVision, UltiMate and SP-100, respectively. Correlation between instruments was highest between FACS and SP-100. This was made possible due to the high repeatability for both instruments. The agreement between the instruments and HEMO as the gold standard was lower than expected as the largest difference in estimation of concentration was -25 to +50%. The largest percentage difference was observed for measurements of dilute semen. It was clear that percentage difference between instruments depended on sperm concentration. In comparison to the gold standard, agreement was highest between SpermVision and HEMO for dilute semen, but for concentrated semen, agreement was highest between SP-100 and HEMO. However, the agreement between HEMO and all other instruments was not as good as expected. The reason may lie within the presence of agglutinated sperm, preventing proper HEMO counts.
当前的研究旨在减少每次输精剂量中的精子数量，因此准确测量原精液中的精子浓度以及生产均匀的输精剂量变得更为关键。本研究评估了使用FACSCount AF系统（FACS）、改良纽鲍尔血细胞计数板（HEMO）、康宁254光度计（Photo C254）、SpermVision计算机辅助精子分析系统（SpermVision）、UltiMate计算机辅助精子分析系统（UltiMate）和NucleoCounter SP-100（SP-100）测定精子浓度的情况。对这些仪器进行了重复性评估，并建立了与HEMO和FACS的回归曲线。FACS、HEMO、Photo C254、SpermVision、UltiMate和SP-100的仪器重复性分别为2.7%、7.1%、10.4%、8.1%、5.4%和3.1%。FACS和SP-100之间的仪器相关性最高。这是由于这两种仪器的高重复性才得以实现。与作为金标准的HEMO相比，其他仪器之间的一致性低于预期，因为浓度估计的最大差异为-25%至+50%。在稀释精液测量中观察到最大百分比差异。很明显，仪器之间的百分比差异取决于精子浓度。与金标准相比，对于稀释精液，SpermVision与HEMO之间的一致性最高，但对于浓缩精液，SP-100与HEMO之间的一致性最高。然而，HEMO与所有其他仪器之间的一致性不如预期。原因可能在于存在凝集精子，妨碍了HEMO的准确计数。
