Abruzzo T, Tun T, Sambanis A
Section of Interventional Neuroradiology, Department of Radiology and The Neuroscience Institute, University of Cincinnati Medical Center, Cincinnati, Ohio, USA.
AJNR Am J Neuroradiol. 2007 Sep;28(8):1586-93. doi: 10.3174/ajnr.A0593.
Endoaneurysmal implantation of fibroblasts may promote healing of aneurysms and reduce recanalization after therapeutic embolization. The purpose of our study was to develop a device for delivery of fibroblasts with use of current microcoil technology.
Cell carrier devices and cell-free devices were fabricated by associating collagen gels (with or without fibroblasts) with platinum microcoils. During the propagation of control cell carrier devices for 1 week in culture, cell-mediated gel contraction (CMGC) occurred. Modified cell carrier devices created by glutaraldehyde cross-linking, ascorbate coculture, or extended CMGC were also characterized in vitro. Devices were deployed through microcatheters (533 microm lumen, 160 cm length). Gel retention, cell retention, cell death, and the ability to support local cell migration were analyzed in vitro.
Cell viability was reduced by glutaraldehyde cross-linking but not by microcatheter transit. During microcatheter transit, cell carrier devices liberated minimal particulate matter and cellular DNA. Liberated particulate matter was reduced by glutaraldehyde cross-linking (P < .05) and extended CMGC (P < .04). Only cell carrier devices treated with glutaraldehyde cross-linking did not exhibit cell migration after microcatheter transit. Passage of cell-free devices through microcatheters sheared off most of their collagen gel.
Collagen gel-platinum microcoil complexes can mediate efficient transmicrocatheter delivery of viable, migration-capable fibroblasts. CMGC is a necessary component of the process of gel stabilization that enables successful microcatheter transit. Although extended CMGC and glutaraldehyde cross-linking enhance gel stabilization, glutaraldehyde cross-linking decreases cell viability and migratory potential.
在动脉瘤内植入成纤维细胞可能促进动脉瘤愈合并减少治疗性栓塞后的再通。本研究的目的是利用当前的微线圈技术开发一种用于递送成纤维细胞的装置。
通过将胶原凝胶（含或不含成纤维细胞）与铂微线圈结合来制造细胞载体装置和无细胞装置。在对照细胞载体装置于培养中传代1周的过程中，发生了细胞介导的凝胶收缩（CMGC）。还对通过戊二醛交联、抗坏血酸共培养或延长CMGC创建的改良细胞载体装置进行了体外特性分析。装置通过微导管（管腔533微米，长度160厘米）进行部署。在体外分析了凝胶保留、细胞保留、细胞死亡以及支持局部细胞迁移的能力。
戊二醛交联降低了细胞活力，但微导管输送未降低细胞活力。在微导管输送过程中，细胞载体装置释放的颗粒物和细胞DNA极少。戊二醛交联（P <.05）和延长CMGC（P <.04）减少了释放的颗粒物。只有经戊二醛交联处理的细胞载体装置在微导管输送后未表现出细胞迁移。无细胞装置通过微导管时，其大部分胶原凝胶被剪切掉。
胶原凝胶 - 铂微线圈复合物可介导有活力、具有迁移能力的成纤维细胞通过微导管有效递送。CMGC是凝胶稳定化过程的必要组成部分，可实现微导管的成功输送。虽然延长CMGC和戊二醛交联增强了凝胶稳定性，但戊二醛交联降低了细胞活力和迁移潜力。
