Seeliger F A, Brügmann M L, Krüger L, Greiser-Wilke I, Verspohl J, Segalés J, Baumgärtner W
Institute for Preclinical Drug Safety, ALTANA Pharma AG, Barsbuettel, Germany.
Vet Pathol. 2007 Sep;44(5):621-34. doi: 10.1354/vp.44-5-621.
Porcine circovirus type 2 (PCV2) is associated with several syndromes in growing pigs, including postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome. In the present study, a previously undescribed neurovascular disorder associated with a PCV2 infection is described. Sixteen pigs showed clinical signs of wasting and neurologic deficits. Acute hemorrhages and edema of cerebellar meninges and parenchyma due to a necrotizing vasculitis resulted in degeneration and necrosis of the gray and white matter. Few to numerous PCV2 DNA and antigen-bearing endothelial cells were detected in affected areas of the brain using in situ hybridization and immunohistochemistry. Conventional histochemical stains, as well as the detection of caspase 3 activity and DNA strand breaks by the terminal transferase dUTP nick end labeling assay, showed numerous apoptotic endothelial cells in the vascular lesions observed. Sequencing of various brain-derived PCV2-specific amplicons revealed a strong identity between different isolates and an 89 to 100% identity to previous isolates. The phylogenetic tree showed that there was no clustering of isolates correlating to clinical signs or geographic distribution. This previously undescribed PCV2-associated neurologic disease has features of both postweaning multisystemic wasting syndrome and, to a lesser extent, porcine dermatitis and nephropathy syndrome. The available evidence suggests that direct virus-induced apoptosis of endothelial cells plays a role in the pathogenesis of this unusual PCV2-associated cerebellar vasculitis.
猪圆环病毒2型（PCV2）与生长猪的多种综合征有关，包括断奶后多系统消耗综合征和猪皮炎肾病综合征。在本研究中，描述了一种与PCV2感染相关的此前未描述的神经血管疾病。16头猪表现出消瘦和神经功能缺损的临床症状。坏死性血管炎导致小脑脑膜和实质急性出血和水肿，进而引起灰质和白质变性和坏死。使用原位杂交和免疫组织化学方法，在受影响的脑区检测到少量至大量含PCV2 DNA和抗原的内皮细胞。传统组织化学染色以及通过末端转移酶dUTP缺口末端标记法检测半胱天冬酶3活性和DNA链断裂，结果显示在观察到的血管病变中有大量凋亡的内皮细胞。对各种源自脑的PCV2特异性扩增子进行测序，结果显示不同分离株之间具有高度一致性，与先前分离株的一致性为89%至100%。系统发育树表明，分离株的聚类与临床症状或地理分布无关。这种此前未描述的与PCV2相关的神经疾病具有断奶后多系统消耗综合征的特征，在较小程度上还具有猪皮炎肾病综合征的特征。现有证据表明，病毒直接诱导的内皮细胞凋亡在这种不寻常的与PCV2相关的小脑血管炎的发病机制中起作用。
