Matsumoto K, Sugiyama M, Ogura R
Department of Medical Biochemistry, Kurume University School of Medicine, Japan.
Photochem Photobiol. 1991 Sep;54(3):389-92. doi: 10.1111/j.1751-1097.1991.tb02032.x.
To understand and characterize non-dimer DNA damage and cytotoxicity induced by ultraviolet-B light (UV-B, 290-320 nm), an alkaline elution technique for analysis of DNA damage was used on Chinese hamster V-79 cells. Ultraviolet-B exposure produced a dose-dependent induction of DNA single strand breaks and DNA-protein crosslinks; however, there was an absence of DNA-DNA interstrand crosslinks. Neither of these types of DNA damage were repaired within a a 24 h incubation of the cells following a single UV-B exposure; rather the damage increased. Using a colony forming assay, we found that UV-B exposure resulted in an increase of cytotoxicity in a dose-dependent fashion. In addition, UV-B exposure inhibited DNA and RNA synthesis. The role of non-dimer DNA damage in the cytotoxicity induced by UV-B is discussed.
为了解和表征紫外线B（UV-B，290 - 320纳米）诱导的非二聚体DNA损伤和细胞毒性，对中国仓鼠V - 79细胞使用了一种用于分析DNA损伤的碱性洗脱技术。紫外线B照射导致DNA单链断裂和DNA - 蛋白质交联呈剂量依赖性诱导；然而，不存在DNA - DNA链间交联。在单次紫外线B照射后的24小时细胞培养中，这两种类型的DNA损伤均未得到修复；相反，损伤增加。使用集落形成试验，我们发现紫外线B照射以剂量依赖性方式导致细胞毒性增加。此外，紫外线B照射抑制DNA和RNA合成。讨论了非二聚体DNA损伤在紫外线B诱导的细胞毒性中的作用。
