Nagarajan V, Borchert T V
Central Research and Development Division, E.I. DuPont de Nemours Inc, Wilmington, DE 19880-0228.
Res Microbiol. 1991 Sep-Oct;142(7-8):787-92. doi: 10.1016/0923-2508(91)90056-g.
The Bacillus amyloliquefaciens levansucrase gene (sacB[BamP]) was engineered in such a way that a heterologous gene could be inserted between the second and third codon of the mature levansucrase. Extracellular levansucrase activity was detected only when the heterologous protein was secreted into the growth medium. A positive selection system to isolate suppressors of signal sequence mutants in Bacillus subtilis has been developed based on the secretion of levansucrase.
解淀粉芽孢杆菌果聚糖蔗糖酶基因（sacB[BamP]）经过改造，使得一个异源基因能够插入到成熟果聚糖蔗糖酶的第二个和第三个密码子之间。只有当异源蛋白分泌到生长培养基中时，才能检测到细胞外果聚糖蔗糖酶活性。基于果聚糖蔗糖酶的分泌，已开发出一种用于分离枯草芽孢杆菌信号序列突变体抑制子的正向选择系统。
