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枯草芽孢杆菌sacB基因的表达会使革兰氏阳性细菌谷氨酸棒杆菌对蔗糖敏感，但对淡紫链霉菌则不然。

Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans.

作者信息

Jäger W, Schäfer A, Pühler A, Labes G, Wohlleben W

机构信息

Department of Genetics, University of Bielefeld, Germany.

出版信息

J Bacteriol. 1992 Aug;174(16):5462-5. doi: 10.1128/jb.174.16.5462-5465.1992.

DOI:10.1128/jb.174.16.5462-5465.1992
PMID:1644774
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC206388/
Abstract

The expression of the structural gene (sacB) encoding Bacillus subtilis levansucrase in two gram-positive soil bacteria, Corynebacterium glutamicum ATCC 13032 and Streptomyces lividans 1326, was investigated. sacB expression in the presence of sucrose is lethal to C. glutamicum but not to S. lividans. While S. lividans secretes levansucrase into the medium, we could show that the enzyme is retained by C. glutamicum cells. Our results imply that the sacB gene can be used as a positive selection system in coryneform bacteria.

摘要

研究了编码枯草芽孢杆菌果聚糖蔗糖酶的结构基因（sacB）在两种革兰氏阳性土壤细菌谷氨酸棒杆菌ATCC 13032和变铅青链霉菌1326中的表达情况。在蔗糖存在下，sacB的表达对谷氨酸棒杆菌是致死性的，但对变铅青链霉菌不是。虽然变铅青链霉菌将果聚糖蔗糖酶分泌到培养基中，但我们能够证明该酶被谷氨酸棒杆菌细胞保留。我们的结果表明，sacB基因可作为棒状杆菌属细菌中的一种正向选择系统。

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2
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本文引用的文献

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[Genetic analysis of sacB, the structural gene of a secreted enzyme, levansucrase of Bacillus subtilis Marburg].[枯草芽孢杆菌马伯格株分泌性酶果聚糖蔗糖酶的结构基因sacB的遗传分析]
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Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences.在鱼腥藻属PCC 7120菌株中使用条件致死基因来筛选双重组体并捕获插入序列。
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