Mueller J P, Mathiopoulos C, Slack F J, Sonenshein A L
Department of Molecular Biology and Microbiology, Tufts University Health Sciences Campus, Boston, MA 02111.
Res Microbiol. 1991 Sep-Oct;142(7-8):805-13. doi: 10.1016/0923-2508(91)90059-j.
Subtractive differential hybridization was used to identify genes in Bacillus subtilis that are induced by nutrient limitation. Several transcription units were identified. They exhibited increased transcription when cells were deprived of certain nutrients, such as glucose, ammonium, or phosphate, or when cells were treated with decoyinine. The genes have been designated dci (for decoyinine-inducible) and gsi (for glucose-starvation-inducible). Using lacZ transcriptional fusions, the dependence of dci and gsi expression on gene products of the sensor and activator classes of bacterial two-component regulatory systems was examined. Transcription of dciA was impaired by a mutation in spoOA, while expression of gsiA was dependent on the early competence genes comP and comA. The implications of these findings are discussed, and a provisional scheme for information flow during the transition phase from growth to sporulation is proposed.
采用消减差异杂交技术来鉴定枯草芽孢杆菌中受营养限制诱导的基因。鉴定出了几个转录单元。当细胞被剥夺某些营养物质，如葡萄糖、铵或磷酸盐时，或者当细胞用脱氧精胍菌素处理时，它们的转录会增加。这些基因被命名为dci（脱氧精胍菌素诱导型）和gsi（葡萄糖饥饿诱导型）。利用lacZ转录融合，研究了dci和gsi表达对细菌双组分调节系统传感器和激活剂类基因产物的依赖性。spoOA突变会损害dciA的转录，而gsiA的表达则依赖于早期感受态基因comP和comA。讨论了这些发现的意义，并提出了从生长到芽孢形成过渡阶段信息流的临时方案。
