1,2 - 二溴乙烷作为细胞转化的引发剂。
1,2-Dibromoethane as an initiating agent for cell transformation.
作者信息
Colacci A, Perocco P, Vaccari M, Da Vià C, Silingardi P, Manzini E, Horn W, Bartoli S, Grilli S
机构信息
Istituyo Nazionale per la Ricerca sul Cancro-Genova, Biotechnology Satellite Unit-Bologna, Italy.
出版信息
Jpn J Cancer Res. 1995 Feb;86(2):168-73. doi: 10.1111/j.1349-7006.1995.tb03035.x.
The two-stage transformation assay increases the sensitivity of cells to chemicals and permits detection of carcinogens acting as initiating agents. 1,2-Dibromoethane, a representative halogenated aliphatic, has been tested in the two-stage BALB/c 3T3 cells transformation test at dosage from 16 microM to 128 microM. This dose range is much lower than those previously found efficient in transforming BALB/c 3T3 cells. Apart from the lowest dose, which induced borderline effects, all the other assayed dosages appeared to induce heritable changes in the target cells. The initiated cells were revealed as fully transformed foci both in the combination with a chronic promoting treatment and also by allowing cells to perform more rounds of cell replication. The results clearly show that 1,2-dibromoethane can act as an initiator of cell transformation.
两阶段转化试验提高了细胞对化学物质的敏感性，并能够检测作为引发剂的致癌物。1,2-二溴乙烷作为一种典型的卤代脂肪烃，已在两阶段BALB/c 3T3细胞转化试验中进行了测试，剂量范围为16微摩尔至128微摩尔。该剂量范围远低于先前发现的能有效转化BALB/c 3T3细胞的剂量。除了最低剂量诱导的临界效应外，所有其他测定剂量似乎都能诱导靶细胞发生可遗传的变化。无论是与慢性促癌处理联合使用，还是让细胞进行更多轮的细胞复制，引发的细胞都表现为完全转化的集落。结果清楚地表明，1,2-二溴乙烷可作为细胞转化的引发剂。
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