Thejass P, Kuttan G
Department of Immunology, Amala Cancer Research Centre, Thrissur, Kerala State, India.
Immunopharmacol Immunotoxicol. 2007;29(2):173-86. doi: 10.1080/08923970701511728.
Effect of sulforaphane on cell-mediated immune response (CMI) was studied in B16F-10 melanoma-induced metastasis-bearing C57BL/6 mice. Administration of sulforaphane significantly enhanced natural killer (NK) cell activity in metastatic tumor-bearing animals (43.17% cell lysis, on day 5) and the activity was observed earlier than in tumor-bearing control animals (maximum of 9.76% cell lysis, on day 9). Antibody-dependent cellular cytotoxicity also was enhanced significantly in metastatic tumor-bearing animals (41.20% cell lysis on day 9) after sulforaphane administration compared with untreated control tumor-bearing animals (maximum of 12.62% cell lysis on day 15). An early antibody-dependent complement-mediated cytotoxicity also was observed in sulforaphane-treated tumor-bearing animals (26% cell lysis, on day 15). Administration of sulforaphane significantly enhanced the production of IL-2 and IFN-gamma in metastatic tumor-bearing animals. In addition, sulforaphane significantly downregulated the serum levels of proinflammatory cytokines such as IL-1beta, IL-6, TNF-alpha, and GM-CSF during metastasis. These data clearly suggest that sulforaphane effectively inhibited the spread of metastatic tumor cells through the stimulation of CMI, upregulation of IL-2 and IFN-gamma, and downregulation of proinflammatory cytokines IL-1beta, IL-6, TNF-alpha, and GM-CSF.
在B16F - 10黑色素瘤诱导转移的C57BL/6小鼠中研究了萝卜硫素对细胞介导免疫反应（CMI）的影响。给予萝卜硫素可显著增强荷转移瘤动物的自然杀伤（NK）细胞活性（第5天细胞裂解率为43.17%），且该活性比荷瘤对照动物出现得更早（第9天细胞裂解率最高为9.76%）。与未处理的荷瘤对照动物相比（第15天细胞裂解率最高为12.62%），给予萝卜硫素后，荷转移瘤动物的抗体依赖性细胞毒性也显著增强（第9天细胞裂解率为41.20%）。在经萝卜硫素处理的荷瘤动物中还观察到早期抗体依赖性补体介导的细胞毒性（第15天细胞裂解率为26%）。给予萝卜硫素可显著增强荷转移瘤动物中IL - 2和IFN - γ的产生。此外，在转移过程中，萝卜硫素可显著下调促炎细胞因子如IL - 1β、IL - 6、TNF - α和GM - CSF的血清水平。这些数据清楚地表明，萝卜硫素通过刺激CMI、上调IL - 2和IFN - γ以及下调促炎细胞因子IL - 1β、IL - 6、TNF - α和GM - CSF，有效抑制了转移瘤细胞的扩散。
