Sheeja K, Kuttan G
Department of Immunology, Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India.
Asian Pac J Cancer Prev. 2010;11(3):723-9.
Effects of Andrographis paniculata extract and its major component, andrographolide, on cell-mediated immune responses in metastatic tumor bearing animals were studied. NK cell mediated target cell lysis was enhanced by the administration of Andrographis paniculata extract (45.0% cell lysis) and andrographolide (40.2% cell lysis) on the 5th day after tumor induction when compared to untreated metastatic tumor bearing animals in which maximum target cell lysis was observed on 11th day (11.4%). Antibody dependent cell-mediated cytotoxicity (ADCC) was also enhanced by treatment with the extract (42.0% cell lysis) and andrographolide (40.2%) in comparison with the untreated case (11.0%). Similarly, the extract (25%) and andrographolide (22%) showed higher ACC activity than the control (14%) and treatment of extract and andrographolide resulted in significant increase in serum IL-2 and TIMP-1 levels. Furthermore, the levels of proinflammatory cytokines such as IL-1ß, IL-6, GM-CSF and TNF-α were effectively reduced by the administration of extract and andrographolide in metastatic tumor bearing animals.
研究了穿心莲提取物及其主要成分穿心莲内酯对荷转移性肿瘤动物细胞介导免疫反应的影响。与未治疗的荷转移性肿瘤动物相比，在肿瘤诱导后第5天给予穿心莲提取物（细胞裂解率45.0%）和穿心莲内酯（细胞裂解率40.2%）可增强NK细胞介导的靶细胞裂解，未治疗的荷转移性肿瘤动物在第11天观察到最大靶细胞裂解率（11.4%）。与未治疗的情况（11.0%）相比，提取物（细胞裂解率42.0%）和穿心莲内酯（40.2%）处理也增强了抗体依赖性细胞介导的细胞毒性（ADCC）。同样，提取物（25%）和穿心莲内酯（22%）显示出比对照（14%）更高的ACC活性，提取物和穿心莲内酯处理导致血清IL-2和TIMP-1水平显著升高。此外，在荷转移性肿瘤动物中，给予提取物和穿心莲内酯可有效降低促炎细胞因子如IL-1β、IL-6、GM-CSF和TNF-α的水平。
