Duan Can-Xing, Wan Jian-Min, Zhai Hu-Qu, Chen Qing, Wang Jian-Kang, Su Ning, Lei Cai-Lin
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
J Econ Entomol. 2007 Aug;100(4):1450-5. doi: 10.1603/0022-0493(2007)100[1450:qtlmor]2.0.co;2.
Laodelphax striatellus Fallén (Homoptera: Delphacidae), is a serious pest in rice, Oryza sativa L., production. A mapping population consisting of 81 recombinant inbred lines (RILs), derived from a cross between japonica' Kinmaze' and indica' DV85' rice, was used to detect quantitative trait loci (QTLs) for the resistance to L. striatellus. Seedbox screening test (SST), antixenosis test, and antibiosis test were used to evaluate the resistance response of the two parents and 81 RILs to L. striatellus at the seedling stage, and composite interval mapping was used for QTL analysis. When the resistance was measured by SST method, two QTLs conferring resistance to L. striatellus were mapped on chromosome 11, namely, Qsbph11a and Qsbph11b, with log of odds scores 2.51 and 4.38, respectively. The two QTLs explained 16.62 and 27.78% of the phenotypic variance in this population, respectively. In total, three QTLs controlling antixenosis against L. striatellus were detected on chromosomes 3, 4, and 11, respectively, accounting for 37.5% of the total phenotypic variance. Two QTLs expressing antibiosis to L. striatellus were mapped on chromosomes 3 and 11, respectively, explaining 25.9% of the total phenotypic variance. The identified QTL located between markers XNpb202 and C1172 on chromosome 11 was detected repeatedly by three different screening methods; therefore, it may be important to confer the resistance to L. striatellus. Once confirmed in other mapping populations, these QTLs should be useful in breeding for resistance to L. striatellus by marker-assisted selection of different resistance genes in rice varieties.
灰飞虱（Laodelphax striatellus Fallén，同翅目：飞虱科）是水稻（Oryza sativa L.）生产中的一种严重害虫。利用一个由81个重组自交系（RIL）组成的作图群体，该群体源自粳稻‘Kinmaze’和籼稻‘DV85’的杂交，来检测水稻对灰飞虱抗性的数量性状位点（QTL）。采用秧田筛选试验（SST）、抗生性试验和栖抗试验，在苗期评估两个亲本和81个RIL对灰飞虱的抗性反应，并采用复合区间作图法进行QTL分析。当用SST法测定抗性时，在第11染色体上定位到两个赋予水稻对灰飞虱抗性的QTL，即Qsbph11a和Qsbph11b，其对数似然值分别为2.51和4.38。这两个QTL分别解释了该群体中16.62%和27.78%的表型变异。总共在第3、4和11染色体上分别检测到3个控制对灰飞虱栖抗的QTL，占总表型变异的37.5%。两个对灰飞虱表现抗生性的QTL分别定位在第3和11染色体上，解释了总表型变异的25.9%。在第11染色体上标记XNpb202和C1172之间鉴定出的QTL，通过三种不同的筛选方法均被重复检测到；因此，它可能对赋予水稻对灰飞虱的抗性很重要。一旦在其他作图群体中得到证实，这些QTL在通过对水稻品种中不同抗性基因进行标记辅助选择来培育抗灰飞虱品种方面应该会很有用。
