State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Weigang 1, Nanjing, China.
Theor Appl Genet. 2011 May;122(8):1591-604. doi: 10.1007/s00122-011-1557-0. Epub 2011 Mar 8.
Rice stripe disease, caused by rice stripe virus (RSV), is one of the most serious diseases in temperate rice-growing areas. In the present study, we performed quantitative trait locus (QTL) analysis for RSV resistance using 98 backcross inbred lines derived from the cross between the highly resistant variety, Kasalath, and the highly susceptible variety, Nipponbare. Under artificial inoculation in the greenhouse, two QTLs for RSV resistance, designated qSTV7 and qSTV11(KAS), were detected on chromosomes 7 and 11 respectively, whereas only one QTL was detected in the same location of chromosome 11 under natural inoculation in the field. The stability of qSTV11(KAS) was validated using 39 established chromosome segment substitution lines. Fine mapping of qSTV11(KAS) was carried out using 372 BC(3)F(2:3) recombinants and 399 BC(3)F(3:4) lines selected from 7,018 BC(3)F(2) plants of the cross SL-234/Koshihikari. The qSTV11(KAS) was localized to a 39.2 kb region containing seven annotated genes. The most likely candidate gene, LOC_Os11g30910, is predicted to encode a sulfotransferase domain-containing protein. The predicted protein encoded by the Kasalath allele differs from Nipponbare by a single amino acid substitution and the deletion of two amino acids within the sulfotransferase domain. Marker-resistance association analysis revealed that the markers L104-155 bp and R48-194 bp were highly correlated with RSV resistance in the 148 landrace varieties. These results provide a basis for the cloning of qSTV11(KAS), and the markers may be used for molecular breeding of RSV resistant rice varieties.
水稻条纹病毒病（RSV）是温带稻作区最严重的病害之一。本研究利用高抗品种 Kasalath 和高感品种 Nipponbare 杂交的 98 个回交自交系，进行了 RSV 抗性的数量性状位点（QTL）分析。在温室人工接种条件下，在第 7 号和第 11 号染色体上分别检测到两个 RSV 抗性 QTL，分别命名为 qSTV7 和 qSTV11(KAS)，而在田间自然接种条件下，仅在第 11 号染色体的同一位置检测到一个 QTL。利用 39 个已建立的染色体片段替换系对 qSTV11(KAS)的稳定性进行了验证。利用 372 个 BC(3)F(2:3)重组体和 399 个从 SL-234/Koshihikari 杂交的 7018 个 BC(3)F(2)植株中选择的 BC(3)F(3:4)系，对 qSTV11(KAS)进行了精细定位。qSTV11(KAS)定位于一个包含七个注释基因的 39.2 kb 区域。最有可能的候选基因 LOC_Os11g30910，预测编码一个磺基转移酶结构域蛋白。Kasalath 等位基因编码的预测蛋白与 Nipponbare 相比，只有一个氨基酸的替换和磺基转移酶结构域内的两个氨基酸的缺失。标记-抗性关联分析表明，标记 L104-155 bp 和 R48-194 bp 与 148 个地方品种的 RSV 抗性高度相关。这些结果为 qSTV11(KAS)的克隆提供了依据，这些标记可用于 RSV 抗性水稻品种的分子育种。
